| Texto completo | |
| Autor(es): |
Sposito, Larissa
;
Fonseca, Diana
;
Carvalho, Suzana Goncalves
;
Sabio, Rafael Miguel
;
Marena, Gabriel Davi
;
Bauab, Tais Maria
;
Meneguin, Andreia Bagliotti
;
Parreira, Paula
;
Martins, M. Cristina L.
;
Chorilli, Marlus
Número total de Autores: 10
|
| Tipo de documento: | Artigo Científico |
| Fonte: | EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS; v. 199, p. 15-pg., 2024-04-15. |
| Resumo | |
Helicobacter pylori ( H. pylori ) is a microorganism directly linked to severe clinical conditions affecting the stomach. The virulence factors and its ability to form biofilms increase resistance to conventional antibiotics, growing the need for new substances and strategies for the treatment of H. pylori infection. The trans -resveratrol (RESV), a bioactive polyphenol from natural sources, has a potential activity against this gastric pathogen. Here, Chitosan nanoparticles (NP) containing RESV (RESV-NP) were developed for H. pylori management. The RESVNP were prepared using the ionic gelation method and characterized by Dynamic Light Scattering (DLS), Nanoparticle Tracking Analysis (NTA) and, Cryogenic Transmission Electron Microscopy (Cryo - TEM). The encapsulation efficiency (EE) and in vitro release rate of RESV were quantified using high -performance liquid chromatography (HPLC). RESV-NP performance against H. pylori was evaluated by the quantification of the minimum inhibitory/bactericidal concentrations (MIC/MBC), time to kill, alterations in H. pylori morphology in its planktonic form, effects against H. pylori biofilm and in an in vitro infection model. RESV-NP cytotoxicity was evaluated against AGS and MKN-74 cell lines and by hemolysis assay. Acute toxicity was tested using Galleria mellonella model assays. RESV-NP showed a spherical shape, size of 145.3 +/- 24.7 nm, polydispersity index (PDI) of 0.28 +/- 0.008, and zeta potential (ZP) of + 16.9 +/- 1.81 mV in DLS, while particle concentration was 3.12 x 10 11 NP/mL (NTA). RESV-NP EE was 72 %, with full release within the first 5 min. In microbiological assays, RESV-NP presented a MIC/MBC of 3.9 mu g/mL, a time to kill of 24 h for complete eradication of H. pylori . At a concentration of 2xMIC (7.8 mu g/mL), RESV-NP completely eradicated the H. pylori biofilm, and in an in vitro infection model, RESV-NP (4xMIC - 15.6 mu g/mL) showed a significant decrease in bacterial load (1 Log 10 CFU/ mL) when compared to the H. pylori J99 control. In addition, they did not demonstrate a toxic character at MIC concentration for both cell lines. The use of the RESV-NP with mucoadhesion profile is an interesting strategy for oral administration of substances targeting gastric disorders, linked to H. pylori infections. (AU) | |
| Processo FAPESP: | 21/11681-5 - Avaliação da atividade de micropartículas contendo nanopartículas de trans- resveratrol contra cepas clínicas de Helicobacter pylori: estudo in vitro |
| Beneficiário: | Larissa Sposito |
| Modalidade de apoio: | Bolsas no Exterior - Estágio de Pesquisa - Doutorado |
| Processo FAPESP: | 19/09597-6 - Micropartículas gastrorretentivas contendo nanopartículas de trans resveratrol aplicadas ao tratamento de infecções por Helicobacter pylori |
| Beneficiário: | Larissa Sposito |
| Modalidade de apoio: | Bolsas no Brasil - Doutorado |