| Texto completo | |
| Autor(es): |
Duarte-Silva, Murillo
;
Parra, Rogerio Serafim
;
Feitosa, Marley Ribeiro
;
Nardini, Viviani
;
Maruyama, Sandra Regina
;
da Rocha, Jose Joaquim Ribeiro
;
Feres, Omar
;
Cardoso, Cristina Ribeiro de Barros
Número total de Autores: 8
|
| Tipo de documento: | Artigo Científico |
| Fonte: | CLINICAL AND EXPERIMENTAL IMMUNOLOGY; v. 217, n. 1, p. 11-pg., 2024-03-22. |
| Resumo | |
Although perianal Crohn's disease (PCD) is highly associated with the exacerbated inflammation, the molecular basis and immunological signature that distinguish patients who present a history of perianal lesions are still unclear. This paper aims to define immunological characteristics related to PCD. In this cross-sectional observational study, we enrolled 20 healthy controls and 39 CD patients. Blood samples were obtained for the detection of plasma cytokines and lipopolysaccharides (LPS). Peripheral blood mononuclear cells (PBMCs) were phenotyped by flow cytometry. Leukocytes were stimulated with LPS or anti-CD3/anti-CD28 antibodies. Our results show that CD patients had augmented plasma interleukin (IL)-6 and LPS. However, their PBMC was characterized by decreased IL-6 production, while patients with a history of PCD produced higher IL-6, IL-8, and interferon-gamma, along with decreased tumor necrosis factor alpha (TNF). CD patients had augmented FoxP3 and cytotoxic T lymphocyte-associated antigen 4 (CTLA-4) regulatory markers, though the PCD subjects presented a significant reduction in CTLA-4 expression. CTLA-4 as well as IL-6 and TNF responses were able to distinguish the PCD patients from those who did not present perianal complications. In conclusion, IL-6, TNF, and CTLA-4 exhibit a distinct expression pattern in CD patients with a history of PCD, regardless of disease activity. These findings clarify some mechanisms involved in the development of the perianal manifestations and may have a great impact on the disease management. Although perianal Crohn's disease (PCD) is highly associated with the exacerbated inflammation, the molecular basis and immunological signature that distinguish patients who present a history of perianal lesions are still unclear. This paper aims to define immunological characteristics related to PCD. These findings clarify some mechanisms involved in the development of the perianal manifestations and may have a great impact on the disease management. Graphical Abstract (AU) | |
| Processo FAPESP: | 17/16328-6 - Leishmaniose Visceral: abordagens genômicas para análise molecular integrada do hospedeiro e do parasito. |
| Beneficiário: | Sandra Regina Costa Maruyama |
| Modalidade de apoio: | Bolsas no Brasil - Jovens Pesquisadores |