Calcium Cross-Linked Cellulose Nanofibrils: Hydrogel Desing for Local and Controlled Nitric Oxide Release

Autor(es)

Mariano, Marcos ; Naseri, Narges ; Nascimento, Diego Magalhaes ; Franqui, Lidiane ; Seabra, Amedea Barozzi ; Mathew, Aji P. ; Bernardes, Juliana Silva

• Tipo de documento: Artigo Científico
• Fonte: ACS APPLIED BIO MATERIALS; v. N/A, p. 11-pg., 2024-11-20.

Resumo

Nitric oxide (NO) holds promise for wound healing due to its antimicrobial properties and role in promoting vasodilation and tissue regeneration. The local delivery of NO to target cells or organs offers significant potential in numerous biomedical applications, especially when NO donors are integrated into nontoxic viscous matrices. This study presents the development of robust cellulose nanofibril (CNF) hydrogels designed to control the release of nitric oxide (NO) generated in situ from a NO-donor molecule (S-nitrosoglutathione, GSNO) obtained from the nitrosation of its precursor molecule glutathione (GSH). CNF, efficiently isolated from sugar cane bagasse, exhibited a high aspect ratio and excellent colloidal stability in water. Although depletion forces could be observed upon the addition of GSH, this effect did not significantly alter the morphology of the CNF network at low GSH concentrations (<20 mM). Ionic cross-linking with Ca2+ resulted in nontoxic and robust hydrogels (elastic moduli ranging from 300 to 3000 Pa) at low CNF solid content. The release rate of NO from GSNO decreased in CNF from 1.61 to 0.40 mmol. L-1<middle dot>h(-1) when the nanofibril content raised from 0.3 to 1.0 wt %. The stabilization effect monitored for 16 h was assigned to hydrogel mesh size, which was easily tailored by modifying the concentration of CNF in the initial suspension. These results highlight the potential of CNF-based hydrogels in biomedical applications requiring a precise NO delivery. (AU)

• Processo FAPESP: 16/22954-4 - Desenvolvimento de materiais preparados a partir de celulose nanofibrilada com potencial aplicação em curativos
• Beneficiário: Juliana da Silva Bernardes
• Modalidade de apoio: Bolsas no Exterior - Pesquisa
• Processo FAPESP: 16/04514-7 - Estudo do uso de nanopartículas de celulose no controle reológico de fluidos complexos
• Beneficiário: Juliana da Silva Bernardes
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOEN - Regular
• Processo FAPESP: 22/14645-2 - Hidrogéis e próteses vasculares liberadores de óxido nítrico para aplicações cardiovasculares
• Beneficiário: Marcelo Ganzarolli de Oliveira
• Modalidade de apoio: Auxílio à Pesquisa - Temático