| Texto completo | |
| Autor(es): |
Furlan, Joao Pedro Rueda
;
Rosa, Rafael da Silva
;
Ramos, Micaela Santana
;
Lopes, Ralf
;
dos Santos, Lucas David Rodrigues
;
Savazzi, Eduardo Angelino
;
Stehling, Eliana Guedes
Número total de Autores: 7
|
| Tipo de documento: | Artigo Científico |
| Fonte: | Environmental Pollution; v. 362, p. 8-pg., 2024-09-13. |
| Resumo | |
The mcr-type gene encodes the main plasmid-mediated mechanism of colistin resistance and has been reported in several bacterial species obtained from different sources. Anthropogenic activities in the environment favor the evolution of antimicrobial resistance. Indeed, mcr-1-positive Escherichia coli strains were susceptible to non-polymyxins antimicrobials, but now emerging as multidrug-resistant (MDR) lineages. In this regard, hundreds of surface water and agricultural soil samples were screened for the presence of E. coli carrying the mcr-type genes and mcr-1-positive strains were subjected to in-depth genomic analysis. Almost all colistin-resistant strains were classified as MDR, highlighting those obtained from soils that showed resistance to extended-spectrum cephalosporins and carbapenems. International and high-risk clones of E. coli were identified, with ST10 and ST1720 shared between water and soil samples. Resistome analysis showed a broad resistome (AMR, metal tolerance, and biocide resistance). The mcr-1.1 and mcr-1.26 allelic variants were detected on IncX4 and IncI2 plasmids. Curiously, mcr-1-positive E. coli strains from agricultural soils harbored plasmid-mediated bla(CTX-M-1), bla(CTX-M-8), or bla(KPC-2) genes. Virulome analysis demonstrated traits of a high putative virulence potential, with the presence of extraintestinal pathogenic E. coli. Comparative analysis revealed the persistence and dissemination of plasmid-mediated antimicrobial resistance genes in genetically diversity E. coli strains at the human-animal-environmental interface. These findings demonstrate a possible emerging AMR trend with the convergence of resistance to colistin and broad-spectrum beta-lactams in environmental-derived E. coli strains. (AU) | |
| Processo FAPESP: | 21/01655-7 - Resistoma, plasmidoma e viruloma de Enterobacterales isoladas do meio ambiente carreando genes mcr-like |
| Beneficiário: | Eliana Guedes Stehling |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 18/01890-3 - Estudo do perfil de resistência, virulência e epidemiológico de Escherichia coli isoladas do meio ambiente |
| Beneficiário: | João Pedro Rueda Furlan |
| Modalidade de apoio: | Bolsas no Brasil - Doutorado |