Toxoplasma gondii isolates: Multi locus RFLP-PCR genotyping from human patients in Sao Paulo State, Brazil identified distinct genotypes

Autor(es)

Ribeiro Ferreira, Isabelle Martins ^[1] ; Vidal, Jose Ernesto ^[2] ; Brandao de Mattos, Cinara de Cassia ^[3] ; de Mattos, Luiz Carlos ^[3] ; Qu, Daofeng ^[4] ; Su, Chunlei ^[5] ; Pereira-Chioccola, Vera Lucia ^[1]

Afiliação do(s) autor(es)

^[1] Inst Adolfo Lutz Registro, Parasitol Lab, BR-01246902 Sao Paulo - Brazil

^[2] Univ Sao Paulo, Serv Extensao Ao Atendimento Pacientes HIV AIDS, Div Molestias Infecciosas & Parasitarias, Hosp Clin, Fac Med, BR-05508 Sao Paulo - Brazil

^[3] Fac Med Sao Jose do Rio Preto, Lab Imunogenet, Sao Jose Do Rio Preto, SP - Brazil

^[4] Zhejiang Univ, Inst Prevent Vet Med, Coll Anim Sci, Hangzhou, Zhejiang - Peoples R China

^[5] Univ Tennessee, Dept Microbiol, Knoxville, TN 37996 - USA

• Tipo de documento: Artigo Científico
• Fonte: Experimental Parasitology; v. 129, n. 2, p. 190-195, OCT 2011.
• Citações Web of Science: 41

Resumo

This study investigated the genetic characteristics of Toxoplasma gondii samples collected from 62 patients with toxoplasmosis in Sao Paulo State, Brazil. DNA samples were isolated from blood, cerebrospinal fluid and amniotic fluids of 25 patients with cerebral toxoplasmosis and AIDS, two patients with acute toxoplasmosis, 12 patients with ocular toxoplasmosis, six newborns with congenital toxoplasmosis and 17 pregnant women with acute infection. Diagnosis of toxoplasmosis was based in clinical, radiological and laboratory features. Genotyping was performed using multilocus PCR-RFLP genetic markers including SAG1, SAG2, 5'- and 3'-SAG2, alt.SAG2, SAG3, BTUB, GRA6, C22-8, c29-2, L358, PK1 and Apico. Among the 62 clinical samples, 20 (32%) were successfully genotyped at eight or more genetic loci and were grouped to three distinct genotypes. Eighteen samples belonged to ToxoDB Genotype \#65 and the other two samples were identified as ToxoDB Genotypes \#6 and \#71, respectively (http://toxodb.org/toxo/). Patients presenting Genotypes \#6 and \#71 had severe and atypical cerebral toxoplasmosis, characterized by diffuse encephalitis without extensive brain lesions. These results indicate that T. gondii Genotype \#65 may have a high frequency in causing human toxoplasmosis in Sao Paulo State, Brazil. This unusual finding highlights the need to investigate the possible association of parasite genotypes with human toxoplasmosis. (C) 2011 Elsevier Inc. All rights reserved. (AU)

• Processo FAPESP: 08/09311-0 - Diagnóstico laboratorial da toxoplasmose com enfoque nas infecções congênita e cerebral
• Beneficiário: Vera Lúcia Pereira Chioccola
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 09/05505-8 - Genotipagem de cepas polimórficas de Toxoplasma gondii provenientes de pacientes com toxoplasmose
• Beneficiário: Isabelle Martins Ribeiro Ferreira
• Modalidade de apoio: Bolsas no Brasil - Doutorado