Analysis of human biological samples using porous graphitic carbon columns and liquid chromatography-mass spectrometry: a review

Autor(es)

Rodrigues, Tais Betoni ; Cunha, Ricardo Leal ; Barci, Paulo Emilio Pereira ; Santos-Neto, alvaro Jose ; Lancas, Fernando Mauro

• Tipo de documento: Artigo Científico
• Fonte: ANALYTICAL AND BIOANALYTICAL CHEMISTRY; v. 416, n. 24, p. 21-pg., 2024-08-19.

Resumo

Liquid chromatography-mass spectrometry (LC-MS) has emerged as a powerful analytical technique for analyzing complex biological samples. Among various chromatographic stationary phases, porous graphitic carbon (PGC) columns have attracted significant attention due to their unique properties-such as the ability to separate both polar and non-polar compounds and their stability through all pH ranges and to high temperatures-besides the compatibility with LC-MS. This review discusses the applicability of PGC for SPE and separation in LC-MS-based analyses of human biological samples, highlighting the diverse applications of PGC-LC-MS in analyzing endogenous metabolites, pharmaceuticals, and biomarkers, such as glycans, proteins, oligosaccharides, sugar phosphates, and nucleotides. Additionally, the fundamental principles underlying PGC column chemistry and its advantages, challenges, and advances in method development are explored. This comprehensive review aims to provide researchers and practitioners with a valuable resource for understanding the capabilities and limitations of PGC columns in LC-MS-based analysis of human biological samples, thereby facilitating advancements in analytical methodologies and biomedical research. (AU)

• Processo FAPESP: 17/02147-0 - Cromatografia líquida em uma gota e seu acoplamento com espectrometria de massas: estratégias instrumentais, desenvolvimento de materiais, automatização e aplicações analíticas
• Beneficiário: Fernando Mauro Lanças
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 23/07159-7 - Síntese, caracterização e emprego de novos sorventes derivados de grafeno em técnicas de separação
• Beneficiário: Fernando Mauro Lanças
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 22/07258-2 - Estudos para aumento da robustez, versatilidade e eficácia para a determinação de alanina, carnosina, catecolaminas, cafeína e seus metabólito em sangue e músculo por LC-MS/MS
• Beneficiário: Taís Betoni Rodrigues
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado