| Texto completo | |
| Autor(es): Mostrar menos - |
Fagundes, Thayssa S. F.
;
Macedo, Arthur L.
;
Rigato, Dhiego B.
;
Do Amaral, Bruno S.
;
Jimenez, Paula Christine
;
Costa-Lotufo, Leticia, V
;
Pereira, Renata F. A.
;
Aguiar-Alves, Fabio
;
Soares, Angelica R.
;
Vasconcelos, Thatyana R. A.
;
Cass, Quezia B.
;
Valverde, Alessandra L.
Número total de Autores: 12
|
| Tipo de documento: | Artigo Científico |
| Fonte: | Anais da Academia Brasileira de Ciências; v. 93, n. 4, p. 10-pg., 2021-01-01. |
| Resumo | |
The extensive marine biodiversity has proved to be a promising source of substances with biomedical potential. In this study, the cytotoxicity of the Brazilian octocoral Phyllogorgia dilatata (Gorgoniidae) was evaluated against two tumor cell lines and three bacterial strains. The methanol/dichloromethane crude extract presented no antibacterial activity up to the highest concentration tested (512 mu g/mL), however it revealed a noteworthy antiproliferative effect against HCT-116 (80%) and MCF-7 (54%) cell lines at 50 mu g/mL. Therefore, guided by the cytotoxic activity, a multistep chemical fractionation of the extract provided the subfraction 5 (PDPH2-5) with IC 50 values of 3.18 and 17.80 mu g/mL against HCT-116 and MCF-7, respectively. The LC-HRMS/MS analysis of PDPH2-5 showed ions of m/z 219.1742 and 219.1743, characterized as (E,E) and (Z,E) germacrone, after a LC-DAD-SPE/NMR analysis of the hexanic fraction and comparisons of NMR data with the literature. Previously reported assessments to the cytotoxic activity of the (E,E)-diastereoisomer disclosed higher IC 50 values than that obtained for the PDPH2-5 fraction, suggesting, herein, a potentiated effect of the diastereoisomeric mixture. Such remark encourage further bioactivity studies with stereoisomer mixtures and reduce the urge for compound isolation. (AU) | |
| Processo FAPESP: | 14/50244-6 - UHPLC acoplado a espectrômetro de massa do tipo híbrido com analisador quadrupolar e time of flight |
| Beneficiário: | Quezia Bezerra Cass |
| Modalidade de apoio: | Auxílio à Pesquisa - Programa Equipamentos Multiusuários |