Exploring Strategic Approaches for Cv FAP Photodecarboxylation through Violet Light Irradiation

Autor(es)

Breda, Gabriela C. ; Franca, Alexandre S. ; de Oliveira, Kleber T. ; Almeida, Rodrigo V. ; de Souza, Rodrigo O. M. A.

• Tipo de documento: Artigo Científico
• Fonte: Journal of the Brazilian Chemical Society; v. 35, n. 8, p. 5-pg., 2024-08-01.

Resumo

In this study, we describe a light -driven photocatalytic decarboxylation of palmitic acid and related fatty acids using Chlorella variabilis fatty acid photodecarboxylase ( Cv FAP). By utilizing violet light emitting diode (LED) light (50 W; 397 nm), we achieved a remarkable conversion efficiency of 99% within just 4 min, surpassing the previous 79% conversion achieved in 60 min using blue LED light (300 W; 439 nm). Importantly, the use of 50 W violet LED light also resulted in a lower enzyme photoinactivation rate when compared to 300 W blue LED. Comparing the whole -cell biocatalyst with the enzymatic extract, we found that the former demonstrated superior catalytic performance and reduced susceptibility to photoinactivation. Furthermore, whole -cell biocatalyst reuse was demonstrated after five sequential batches. Employing this approach, we successfully synthesized 26 mmol L -1 h -1 of pentadecane, showcasing a promising strategy to improve productivity. These findings represent a significant advancement in Cv FAP photodecarboxylation processes compared to the literature, utilizing an alternative light source, with potential implications to the biofuel sector. (AU)

• Processo FAPESP: 13/07276-1 - CEPOF - Centro de Pesquisa em Óptica e Fotônica
• Beneficiário: Vanderlei Salvador Bagnato
• Modalidade de apoio: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs
• Processo FAPESP: 20/06874-6 - Aplicações das metodologias e tecnologias de síntese em regime de fluxo contínuo desenvolvidas no LQBO-UFSCar na preparação de moléculas com atividades biológicas e IFAs
• Beneficiário: Kleber Thiago de Oliveira
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 19/27176-8 - Fotocatálise assimétrica aplicada à síntese do medicamento Brivaracetam: abordagens sintéticas empregando tecnologias facilitadoras de síntese química
• Beneficiário: Kleber Thiago de Oliveira
• Modalidade de apoio: Auxílio à Pesquisa - Regular