| Texto completo | |
| Autor(es): |
Torres, Mariana de Almeida
;
Jones, Martin R.
;
vom Berg, Colette
;
Pinto, Ernani
;
Janssen, Elisabeth M. -L.
Número total de Autores: 5
|
| Tipo de documento: | Artigo Científico |
| Fonte: | AQUATIC TOXICOLOGY; v. 263, p. 11-pg., 2023-10-01. |
| Resumo | |
Cyanobacterial blooms affect aquatic ecosystems across the globe and one major concern relates to their toxins such as microcystins (MC). Yet, the ecotoxicological risks, particularly non-lethal effects, associated with other co-produced secondary metabolites remain mostly unknown. Here, we assessed survival, morphological alter-ations, swimming behaviour and cardiovascular functions of zebrafish (Danio rerio) upon exposure to cyano-bacterial extracts of two Brazilian Microcystis strains. We verified that only MIRS-04 produced MCs and identified other co-produced cyanopeptides also for the MC non-producer NPCD-01 by LC-HRMS/MS analysis. Both cya-nobacterial extracts, from the MC-producer and non-producer, caused acute toxicity in zebrafish with LC50 values of 0.49 and 0.98 mgdw_biomass/mL, respectively. After exposure to MC-producer extract, additional decreased locomotor activity was observed. The cyanopeptolin (micropeptin K139) contributed 52% of the overall mor-tality and caused oedemas of the pericardial region. Oedemas of the pericardial area and prevented hatching were also observed upon exposure to the fraction with high abundance of a microginin (Nostoginin BN741) in the extract of the MC non-producer. Our results further add to the yet sparse understanding of lethal and sublethal effects caused by cyanobacterial metabolites other than MCs and the need to better understand the underlying mechanisms of the toxicity. We emphasize the importance of considering mixture toxicity of co-produced metabolites in the ecotoxicological risk assessment of cyanobacterial bloom events, given the importance for predicting adverse outcomes in fish and other organisms. (AU) | |
| Processo FAPESP: | 21/00149-0 - Tratamento e fracionamento seletivo de cianobactérias em florações para produção de insumos de valor agregado |
| Beneficiário: | Ernani Pinto Junior |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |