MMP-2 and MMP-9 localization and activity in the female prostate during estrous cycle

Autor(es)

Santos, Fernanda C. A. ^[1] ; Rochel-Maia, Sabrina S. ^[2] ; Fochi, Ricardo A. ^[2] ; Justulin, Jr., Luis A. ^[3] ; Santos, Sergio A. A. ^[4] ; Vilamaior, Patricia S. L. ^[5] ; Felisbino, Sergio L. ^[4] ; Goes, Rejane M. ^[6] ; Taboga, Sebastiao R. ^[6]

Afiliação do(s) autor(es)

^[1] Fed Univ Goias UFG, Inst Biol Sci, Dept Morphol, BR-74001970 Goiania, Go - Brazil

^[2] Univ Estadual Campinas, UNICAMP, Inst Biol, Dept Anat Cell Biol Physiol & Biophys, BR-1308486 Sao Paulo - Brazil

^[3] Fed Univ Triangulo Mineiro UFTM, Dept Morphol, BR-38025000 Uberaba, MG - Brazil

^[4] Univ Estadual Paulista, UNESP, Dept Morphol, Inst Biosci IBB, BR-18618000 Sao Paulo - Brazil

^[5] Rio Preto Univ Ctr UNIRP, Biol Sci & Vet Med Sch, BR-15025400 Sao Paulo - Brazil

^[6] Univ Estadual Paulista, UNESP, Dept Biol, Inst Biosci Humanities & Exact Sci IBILCE, BR-15054000 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: General and Comparative Endocrinology; v. 173, n. 3, p. 419-427, SEP 15 2011.
• Citações Web of Science: 8

Resumo

The gerbil female prostate undergoes morphological and physiological changes resulting from hormonal fluctuations that occur during the reproductive cycle. These repetitive cycles of glandular growth and regression are followed by an extensive reconstruction and remodeling of prostate stroma throughout the reproductive life of the female gerbil. The objective of this study was to evaluate the effect that the hormonal fluctuations of the reproductive cycle have on the stromal remodeling and the expression and activity of matrix metalloproteinases MMP-2 and-gin the adult female gerbil prostate. For this, serological, ultrastructural, immunohistochemical and biochemical methods were employed. The results showed that the major stromal alteration coincide with the peak of estradiol, which occurs in estrus, and with the peak of progesterone, occurring during diestrus II. MMP-2 and -9 presented a similar pattern of expression and activity during estrous cycle. The estrus was the phase of greater expression and activity of MMP-2 and -9. On the other hand, in DI and DII, the tissue expression and activity of MMP-2 and -9 was very weak. These results are important since they suggest the involvement of estradiol and progesterone in regulating the expression and activity of MMP-2 and -9 in adult gerbil female prostate. (C) 2011 Elsevier Inc. All rights reserved. (AU)

• Processo FAPESP: 06/06952-0 - Aspectos morfofuncionais da prostata feminina do gerbilo durante o ciclo estral: avaliacao imuno-histoquimica, ultra-estrutural e bioquimica.
• Beneficiário: Fernanda Cristina Alcantara dos Santos
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 06/06985-5 - Modulação androgênica da expressão de MMP-2 e MMP-9 e serino proteases na próstata masculina e feminina de gerbilos
• Beneficiário: Sabrina Santos Rochel Maia
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 06/06876-1 - Aspectos morfofuncionais da prostata feminina do gerbilo durante o ciclo estral.
• Beneficiário: Sebastião Roberto Taboga
• Modalidade de apoio: Auxílio à Pesquisa - Regular