KPC-2-producing Enterobacterales from ready-to-eat food to hospitalized patients1

Autor(es)

de Godoy, Bianca Lara Venancio ; Barroso, Marlon do Valle ; Lourenca, Yasmin Fontoura de Azeredo ; de Andrade, Leticia Kellen ; Rodrigues, Vitoria Gabriela Tosta ; Atui, Caroline ; do Valle, Ana Caroline ; Ferreira, Tais Paulino ; Nogueira, Mara Correa Lelles ; Casella, Tiago

• Tipo de documento: Artigo Científico
• Fonte: INFECTION GENETICS AND EVOLUTION; v. 121, p. 6-pg., 2024-04-25.

Resumo

Foodstuffs are a well-documented source of multidrug-resistant bacteria, and hospitalized patients are usually susceptible to hospital infections owing to their immune status. Therefore, this study aimed to investigate the presence of beta-lactamase-producing Enterobacterales in ready-to-eat foods consumed by hospitalized patients. For this purpose, 51 vegetable and meat samples were collected over 2 months and analyzed. Enterobacterales isolates were identified and subjected to antimicrobial susceptibility testing, followed by beta-lactamase gene screening, pH tolerance assays, and whole-genome sequencing (WGS). Isolates harboring genes encoding extended-spectrum beta-lactamases, cephalosporinases, or carbapenemases were detected, and all isolates tolerated pH levels similar to those in the human gastrointestinal tract. The blaKPC-2 carriers were characterized by WGS and lineages closely related to those causing human infections were identified. These results showed that dietary intake is an alternative route for the transmission of antimicrobial-resistant bacteria, which must be considered when designing effective strategies for infection control. (AU)

• Processo FAPESP: 23/02640-9 - Similaridade genética e produção de biofilme em isolados de Klebsiella pneumoniae produtores de KPC obtidos de infecção e de colonização
• Beneficiário: Caroline Atuí
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 23/02642-1 - Caracterização de Enterobacteriaceae resistentes a cefalosporinas de amplo espectro isoladas de alimentos servidos a pacientes hospitalizados
• Beneficiário: Vitória Gabriela Tosta Rodrigues
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 20/08482-8 - Clones de alto risco de Klebsiella pneumoniae resistentes a ß-lactâmicos de amplo espectro: uma perspectiva em Saúde Única
• Beneficiário: Tiago Casella
• Modalidade de apoio: Auxílio à Pesquisa - Regular