| Texto completo | |
| Autor(es): |
Batista, Raquel Salgado
;
Chaves, Gabriel Luz
;
Oliveira, Davi Benedito
;
Pantalea, Vitor Leonel
;
Neves, Jose Davi dos Santos
;
da Silva, Adilson Jose
Número total de Autores: 6
|
| Tipo de documento: | Artigo Científico |
| Fonte: | Bioresource Technology; v. 393, p. 10-pg., 2023-12-12. |
| Resumo | |
Microbial engineering is a promising way to produce 3-HP using biorenewable substrates such as glycerol. However, the glycerol pathway to obtain 3-HP requires vitamin B-12, which hinders its economic viability. The present work showed that 3-HP can be efficiently produced from glycerol through the 8-alanine pathway. To develop a cell factory for this purpose, glycerol was evaluated as a substrate and showed more than two-fold improved 3-HP production compared to glucose. Next, the reducing power was modulated by overexpression of an NADP+-dependent glyceraldehyde-3-phosphate dehydrogenase coupled with CRISPR-based repression of the endogenous gapA gene, resulting in a 91 % increase in 3-HP titer. Finally, the toxicity of 3-HP accumulation was addressed by overexpressing a putative exporter (YohJK). Fed-batch cultivation of the final strain yielded 72.2 g/L of 3-HP and a productivity of 1.64 g/L/h, which are the best results for the 8-alanine pathway and are similar to those found for other pathways. (AU) | |
| Processo FAPESP: | 16/10636-8 - Da fábrica celular à biorrefinaria integrada Biodiesel-Bioetanol: uma abordagem sistêmica aplicada a problemas complexos em micro e macroescalas |
| Beneficiário: | Roberto de Campos Giordano |
| Modalidade de apoio: | Auxílio à Pesquisa - Programa BIOEN - Temático |
| Processo FAPESP: | 22/07833-7 - Avaliação de alvos genéticos potenciais para otimização da produção de fenazinas em E. coli |
| Beneficiário: | Vitor Leonel Pantaleão |
| Modalidade de apoio: | Bolsas no Brasil - Iniciação Científica |
| Processo FAPESP: | 19/07902-6 - Construção de uma fábrica celular para produção de ácido 3-hidroxipropiônico |
| Beneficiário: | Gabriel Luz Chaves |
| Modalidade de apoio: | Bolsas no Brasil - Mestrado |