Leucine supplementation improves adiponectin and total cholesterol concentrations despite the lack of changes in adiposity or glucose homeostasis in rats previously exposed to a high-fat diet

Autor(es)

Torres-Leal, Francisco L. ^[1] ; Fonseca-Alaniz, Miriam H. ^[2] ; Teodoro, Gabriela F. R. ^[1] ; de Capitani, Mariana D. ^[1] ; Vianna, Daiana ^[1] ; Pantaleao, Lucas C. ^[1] ; Matos-Neto, Emidio M. ^[1] ; Rogero, Marcelo M. ^[3] ; Donato, Jr., Jose ^[4] ; Tirapegui, Julio ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Pharmaceut Sci, Dept Food Sci & Expt Nutr, Sao Paulo - Brazil

^[2] Univ Sao Paulo, Sch Med, Heart Inst InCor, Sao Paulo - Brazil

^[3] Univ Sao Paulo, Sch Publ Hlth, Dept Nutr, Sao Paulo - Brazil

^[4] Univ Texas SW Med Ctr Dallas, Div Hypothalam Res, Dept Internal Med, Dallas, TX 75390 - USA

• Tipo de documento: Artigo Científico
• Fonte: NUTRITION & METABOLISM; v. 8, SEP 7 2011.
• Citações Web of Science: 33

Resumo

Background: Studies suggest that leucine supplementation (LS) has a therapeutic potential to prevent obesity and to promote glucose homeostasis. Furthermore, regular physical exercise is a widely accepted strategy for body weight maintenance and also for the prevention of obesity. The aim of this study was to determine the effect of chronic LS alone or combined with endurance training (ET) as potential approaches for reversing the insulin resistance and obesity induced by a high-fat diet (HFD) in rats. Methods: Forty-seven rats were randomly divided into two groups. Animals were fed a control diet-low fat (n = 10) or HFD (n = 37). After 15 weeks on HFD, all rats received the control diet-low fat and were randomly divided according to treatment: reference (REF), LS, ET, and LS+ET (n = 7-8 rats per group). After 6 weeks of treatment, the animals were sacrificed and body composition, fat cell volume, and serum concentrations of total cholesterol, HDL-cholesterol, triacylglycerol, glucose, adiponectin, leptin and tumor necrosis factor-alpha (TNF-alpha) were analyzed. Results: At the end of the sixth week of treatment, there was no significant difference in body weight between the REF, LS, ET and LS+ET groups. However, ET increased lean body mass in rats (P = 0.019). In addition, ET was more effective than LS in reducing adiposity (P = 0.019), serum insulin (P = 0.022) and TNF-alpha (P = 0.044). Conversely, LS increased serum adiponectin (P = 0.021) levels and reduced serum total cholesterol concentration (P = 0.042). Conclusions: The results showed that LS had no beneficial effects on insulin sensitivity or adiposity in previously obese rats. On the other hand, LS was effective in increasing adiponectin levels and in reducing total cholesterol concentration. (AU)

• Processo FAPESP: 07/51964-9 - Suplementacao com leucina nao reproduz os efeitos beneficos do exercicio fisico sobre a adiposidade e a homeostase glicemica de ratos apos dieta hiperlipidica.
• Beneficiário: Francisco Leonardo Torres Leal
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 07/56230-3 - Efeitos da suplementação crônica com leucina na composição corporal durante o envelhecimento: estudos em ratos
• Beneficiário: Julio Orlando Tirapegui Toledo
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 08/58428-8 - Efeitos da dieta hiperlipidica sobre o ganho de peso corporal, adiposidade e adipocinas em ratos propensos e resistentes a obesidade.
• Beneficiário: Mariana Dutilh de Capitani
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica