| Texto completo | |
| Autor(es): |
de Oliveira, Daniele Goncalves
;
Mazziero, Vitor Teixeira
;
Picao, Bruno Willian
;
Ribeiro, Renata Maria de Magalhaes Gomes Pontes
;
Esperanca, Mateus Nordi
;
de Paula, Ariela Veloso
;
Cerri, Marcel Otavio
Número total de Autores: 7
|
| Tipo de documento: | Artigo Científico |
| Fonte: | Biochemical Engineering Journal; v. 207, p. 13-pg., 2024-04-12. |
| Resumo | |
Airlift bioreactors present a compelling alternative for reactions utilizing immobilized enzymes, offering advantages over stirred tank and packed -bed reactors. This study proposed a new configuration for an internal -loop airlift bioreactor (1 L working volume) to produce structured triglycerides using immobilized lipases in a viscous fluid. Different internal tube configurations were studied, which varied in diameter (17.5-78.6 mm), angle (-2,0,5,10 and 12 degrees), and the addition of a rounded bottom to the bioreactor, which improved the three-phase mixture flow. The hydrodynamic effect caused by these modifications was evaluated by studying the mixing time. The best internal tube configuration, featuring a truncated cone -shaped, exhibited a 5 degrees angle and a 64.5mm diameter, resulting in a mixing time of 54 s. This value was about three times less than the mixing time obtained by the standard tube (171 s) at 2 vvm. Sequentially, grape seed oil acidolysis reactions with capric acid (C10) catalyzed by the Lipozyme RM IM were performed, obtaining structured MLM triglycerides with an incorporation degree of 32.32% +/- 1.01%. These results indicated good biocatalyst operational stability, with a half-life of 14.13 days. Thus, this work unveils a new configuration for an airlift bioreactor collectively driving promising advancements in structured lipid production. (AU) | |
| Processo FAPESP: | 20/08699-7 - Melhoria de desempenho em biorreator Airlift de circulação interna através de modificações geométricas no tubo interno |
| Beneficiário: | Marcel Otavio Cerri |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 17/11482-7 - Síntese enzimática de triglicerídeos dietéticos empregando reatores do tipo Airlift, leito fixo e leito fluidizado |
| Beneficiário: | Ariela Veloso de Paula |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 20/09592-1 - Ésteres bioativos nanoencapsulados: síntese enzimática e estudo do potencial antimicrobiano |
| Beneficiário: | Ariela Veloso de Paula |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |