Characterization of the bacterial microbiome of non-hematophagous bats and associated ectoparasites from Brazil

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Andre, Marcos Rogerio ; Ikeda, Priscila ; Lee, Daniel Antonio Braga ; do Amaral, Renan Bressianini ; Carvalho, Lucas Amoroso Lopes ; Pinheiro, Daniel Guariz ; Torres, Jaire Marinho ; de Mello, Victoria Valente Califre ; Rice, Gregory K. ; Cer, Regina Z. ; Lourenco, Elizabete Captivo ; Oliveira, Carisa Elisei ; Herrera, Heitor Miraglia ; Barros-Battesti, Darci Moraes ; Machado, Rosangela Zacarias ; Bishop-Lilly, Kimberly A. ; Dalgard, Clifton L. ; Dumler, J. Stephen

• Tipo de documento: Artigo Científico
• Fonte: FRONTIERS IN MICROBIOLOGY; v. 14, p. 15-pg., 2023-10-19.

Resumo

IntroductionBats, along with their ectoparasites, harbor a wide diversity of symbiotic and potential pathogenic bacteria. Despite the enormous diversity of bats (181 species), few studies aimed to investigate the bacterial microbiome of Brazilian chiropterans and associated ectoparasites. This study aimed to characterize the bacterial microbiome of non-hematophagous bats and associated Streblidae flies and Macronyssidae and Spinturnicidae mites in the state of Mato Grosso do Sul, midwestern Brazil.MethodsOral and rectal swabs were collected from 30 bats (Artibeus lituratus [n = 13], Artibeus planirostris [n = 9], Eptesicus furinalis [n = 5], Carollia perspicillata [n = 2], and Platyrrhinus lineatus [n = 1]). In addition, a total of 58 mites (15 Macronyssidae and 43 Spinturnicidae) and 48 Streblidae bat flies were collected from the captured bats. After DNA extraction and purification, each sample's bacterial composition was analyzed with metagenomic sequencing.ResultsThe microbiome composition of both oral and rectal bat swab samples showed that Gammaproteobacteria was the most abundant bacterial class. Spiroplasma, Wolbachia and Bartonella represented the most abundant genera in Streblidae flies. While Wolbachia (Alphaproteobacteria) was the most abundant genus found in Spinturnicidae, Arsenophonus (Gammaproteobacteria) was found in high abundance in Macronyssidae mites. In addition to characterizing the microbiome of each sample at the class and genus taxonomic levels, we identified medically significant bacteria able to infect both animals and humans in oral (Streptococcus and Anaplasma) and rectal swabs (Enterobacter, Klebsiella, Escherichia, Enterococcus, Streptococcus), Macronyssidae (Anaplasma, Bartonella, Ehrlichia) and Spinturnicidae (Anaplasma, Bartonella) mites as well as Streblidae flies (Spiroplasma, Bartonella).Discussion and conclusionBesides expanding the knowledge on the bacterial microbiome of non-hematophagous bats and Streblidae flies from Brazil, the present work showed, for the first time, the bacterial community of bat-associated Macronyssidae and Spinturnicidae mites. (AU)

• Processo FAPESP: 22/05615-2 - Investigação da relação entre Bartonella spp. e artrópodes vetores por meio de técnicas omics e de cultivo em linhagens celulares de carrapatos
• Beneficiário: Marcos Rogério André
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 18/19672-2 - ISOLAMENTO, GENOTIPAGEM E SEQUENCIAMENTO GENÔMICO DE Bartonella spp. EM PEQUENOS MAMÍFEROS SELVAGENS E RUMINANTES NO BRASIL
• Beneficiário: Renan Bressianini do Amaral
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 22/07008-6 - Detecção e caracterização molecular de Bartonella spp. em flebotomíneos (Diptera: Psychodidae) nas regiões norte e nordeste do Brasil
• Beneficiário: Daniel Antônio Braga Lee
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 19/13232-3 - Microbioma bacteriano de ectoparasitas, saliva e fezes de morcegos não hematófagos: uma abordagem metagenômica
• Beneficiário: Priscila Ikeda
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Doutorado