| Texto completo | |
| Autor(es): |
de Carvalho, Gabriel Garcia
;
Vieira, Basilio Rodrigues
;
Carvalho, Jhonatan de Souza
;
de Sousa, Frederico Barbosa
;
Cerri, Paulo Sergio
;
de Oliveira, Kleber Thiago
;
Chorilli, Marlus
;
Zandim-Barcelos, Daniela Leal
;
Spolidorio, Luis Carlos
;
Spolidorio, Denise Madalena Palomari
Número total de Autores: 10
|
| Tipo de documento: | Artigo Científico |
| Fonte: | Photodiagnosis and Photodynamic Therapy; v. 49, p. 9-pg., 2024-09-23. |
| Resumo | |
Background: The combination of photodynamic therapy (PDT) and LL-37 has never been tested in an animal study and our research team background suggests this strategy might be a promising alternative to intensify periodontitis resolution. This study aimed to assess the effects of multiple sessions of PDT with chlorin-e6 conjugated to the antimicrobial peptide LL-37 loaded nanoemulsion, as adjunctive therapy in experimental periodontitis in rats. Methods: Experimental periodontitis was induced in 81 rats. After disease establishment, animals were assigned to three groups: SRP (scaling and root planning); SRP + 1PDT, SRP followed by a single PDT session; SRP + 4PDT (n n = 27), SRP followed by four PDT sessions at 0, 24, 48 and 72 h after SRP. Animals were subjected to euthanasia at 7, 14 and 28 days, and samples were submitted to osteoclast quantification, immunological and microtomography analysis. Results: All treatments resulted in significant periodontal improvements and there was no significant difference between the groups in both local inflammatory response and healing process. Minimal adjunctive effects could be found for the combined therapy in terms of cytokine levels (IL-1 beta and IL-10), with no statistical significance. However, the number of TRAP-positive osteoclasts per mm of alveolar bone linear surface for the group treated with PDT sessions was significantly lower than those treated with SRP only. Conclusions: Multiple PDT sessions with chlorin-e6 and LL-37 nanoemulsion as an adjunct to scaling and root planning reduced the presence of osteoclast in the local site but did not contribute towards bone regeneration and IL-1 beta and IL-10 levels. (AU) | |
| Processo FAPESP: | 13/07276-1 - CEPOF - Centro de Pesquisa em Óptica e Fotônica |
| Beneficiário: | Vanderlei Salvador Bagnato |
| Modalidade de apoio: | Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs |
| Processo FAPESP: | 20/04499-3 - Terapia fotodinâmica antimicrobiana com utilização da clorina-e6 em sistema de nanoemulsão combinado a peptídeo antimicrobiano no tratamento da doença periodontal |
| Beneficiário: | Gabriel Garcia de Carvalho |
| Modalidade de apoio: | Bolsas no Brasil - Doutorado |
| Processo FAPESP: | 23/04020-8 - Síntese de IFAs utilizando-se tecnologias facilitadoras: abordagens envolvendo eletrossíntese e fotocatálise em regime de fluxo contínuo |
| Beneficiário: | Kleber Thiago de Oliveira |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 18/09088-1 - Avaliação da terapia fotodinâmica contra biofilmes acneicos polimicrobianos: um estudo pré-clínico |
| Beneficiário: | Sarah Raquel de Annunzio |
| Modalidade de apoio: | Bolsas no Brasil - Doutorado |
| Processo FAPESP: | 21/05881-1 - Desenvolvimento e avaliação de nanofibras e do carreador lipídico nanoestruturado associados à hesperitina como potencial terapêutico no reparo dos tecidos periodontais |
| Beneficiário: | Denise Madalena Palomari Spolidorio |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |