| Texto completo | |
| Autor(es): |
Silva, Jessika C. A.
;
Marques-Neto, Lazaro M.
;
Carvalho, Eneas
;
Del Carpio, Alejandra M. G.
;
Henrique, Camila
;
Leite, Luciana C. C.
;
Mitsunari, Thais
;
Elias, Waldir P.
;
Munhoz, Danielle D.
;
Piazza, Roxane M. F.
Número total de Autores: 10
|
| Tipo de documento: | Artigo Científico |
| Fonte: | TOXINS; v. 16, n. 11, p. 15-pg., 2024-11-01. |
| Resumo | |
The functions of bacterial plasmid-encoded toxin-antitoxin (TA) systems are unambiguous in the sense of controlling cells that fail to inherit a plasmid copy. However, its role in chromosomal copies is contradictory, including stress-response-promoting fitness and antibiotic treatment survival. A hybrid pathogenic Escherichia coli strain may have the ability to colonize distinct host niches, facing contrasting stress environments. Herein, we determined the influence of multiple environmental stress factors on the bacterial growth dynamic and expression profile of previously described TA systems present in the chromosome of a hybrid atypical enteropathogenic and extraintestinal E. coli strain. Genomic analysis revealed 26 TA loci and the presence of five type II TA systems in the chromosome. Among the tested stress conditions, osmotic and acid stress significantly altered the growth dynamics of the hybrid strain, enhancing the necessary time to reach the stationary phase. Using qPCR analyses, 80% of the studied TA systems were differentially expressed in at least one of the tested conditions, either in the log or in the stationary phase. These data indicate that type II TA systems may contribute to the physiology of pathogenic hybrid strains, enabling their adaptation to different milieus. (AU) | |
| Processo FAPESP: | 17/14821-7 - Explorando novas estratégias de virulência em Escherichia coli |
| Beneficiário: | Tânia Aparecida Tardelli Gomes do Amaral |
| Modalidade de apoio: | Auxílio à Pesquisa - Temático |
| Processo FAPESP: | 19/02305-0 - Investigação de mecanismos de resposta imune efetora de uma vacina de BCG recombinante contra Tuberculose por Systems Biology |
| Beneficiário: | Lázaro Moreira Marques Neto |
| Modalidade de apoio: | Bolsas no Brasil - Pós-Doutorado |
| Processo FAPESP: | 17/24832-6 - Desenvolvimento de vacinas baseadas em BCG recombinante: Tuberculose, Pertussis, Pneumococo e Schistosoma |
| Beneficiário: | Luciana Cezar de Cerqueira Leite |
| Modalidade de apoio: | Auxílio à Pesquisa - Temático |
| Processo FAPESP: | 23/01700-8 - Papel do sistema toxina-antitoxina tipo II na patogênese bacteriana de uma cepa híbrida: Escherichia coli enteropatogênica atípica e E. coli extraintestinal |
| Beneficiário: | Jessika Cristina Alves da Silva |
| Modalidade de apoio: | Bolsas no Brasil - Doutorado |