Uncovering the Association Mechanism between Two Intrinsically Flexible Proteins

Autor(es)

Davalos, Angy Liseth ; Echeverri, Jose David Rivera ; Favaro, Denize C. ; de Oliveira, Ronaldo Junio ; Carretero, Gustavo Battesini ; Lacerda, Caroline ; Cuccovia, Iolanda Midea ; Cardoso, Marcus Vinicius Cangussu ; Farah, Chuck S. ; Salinas, Roberto Kopke

• Tipo de documento: Artigo Científico
• Fonte: ACS Chemical Biology; v. 19, n. 3, p. 18-pg., 2024-03-05.

Resumo

The understanding of protein-protein interaction mechanisms is key to the atomistic description of cell signaling pathways and for the development of new drugs. In this context, the mechanism of intrinsically disordered proteins folding upon binding has attracted attention. The VirB9 C-terminal domain (VirB9(Ct)) and the VirB7 N-terminal motif (VirB7(Nt)) associate with VirB10 to form the outer membrane core complex of the Type IV Secretion System injectisome. Despite forming a stable and rigid complex, VirB7(Nt) behaves as a random coil, while VirB9(Ct) is intrinsically dynamic in the free state. Here we combined NMR, stopped-flow fluorescence, and computer simulations using structure-based models to characterize the VirB9(Ct)-VirB7(Nt) coupled folding and binding mechanism. Qualitative data analysis suggested that VirB9(Ct) preferentially binds to VirB7(Nt) by way of a conformational selection mechanism at lower temperatures. However, at higher temperatures, energy barriers between different VirB9(Ct) conformations are more easily surpassed. Under these conditions the formation of non-native initial encounter complexes may provide alternative pathways toward the native complex conformation. These observations highlight the intimate relationship between folding and binding, calling attention to the fact that the two molecular partners must search for the most favored intramolecular and intermolecular interactions on a rugged and funnelled conformational energy landscape, along which multiple intermediates may lead to the final native state. (AU)

• Processo FAPESP: 18/21450-8 - Estudos de Cryo-EM sobre os sistemas de secreção Tipo IV Xanthomonadaceae
• Beneficiário: Jose David Rivera Echeverri
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 16/17375-5 - Estudos de dinâmica de proteínas por ressonância magnética nuclear em solução
• Beneficiário: Marcus Vinícius Cangussu Cardoso
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 17/17303-7 - Estrutura e função de sistemas de secreção bacterianas
• Beneficiário: Shaker Chuck Farah
• Modalidade de apoio: Auxílio à Pesquisa - Temático