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Effect of Blue LED Light on Bioemulsifier Production in Bioreactor by Aureobasidium pullulans LB83 in Solid State Fermentation

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Autor(es):
Rubio-Ribeaux, Daylin ; da Costa, Rogger Alessandro Mata ; Pereira, Renan Murbach ; Marcelino, Paulo Ricardo Franco ; Casciatori, Fernanda Perpetua ; dos Santos, Julio Cesar ; da Silva, Silvio Silverio
Número total de Autores: 7
Tipo de documento: Artigo Científico
Fonte: FERMENTATION-BASEL; v. 9, n. 11, p. 15-pg., 2023-11-01.
Resumo

This study analyzed the impact of LED light on bioemulsifier production by Aureobasidium pullulans LB83 in solid-state fermentation (SSF) using pre-treated sugarcane bagasse (PSB). The biomass was subjected to alkaline pre-treatment and conducted fermentations in Erlenmeyer flasks containing 2 g of PSB that were immersed in a humectant solution with a cell concentration of 10(8) cells/mL. The screening involved varying LED light wavelengths (green, red, orange, and blue) over a 7-day period at 28 degrees C. Notably, under the influence of blue light, the process achieved maximum production, yielding an EI24% of 63.9% and 45.1% for soybean oil and kerosene, respectively. Prolonged exposure to blue light for 11 days at 28 degrees C resulted in maximum bioemulsifier production (75%) and cellulolytic enzyme activity (3.67 IU g(-1) for endoglucanase and 0.41 IU g(-1) for exoglucanase) with soybean oil and kerosene. Experiments in a bioreactor, with varying light conditions (dark, white light, and blue LED light), demonstrated that the blue LED bioreactor outperformed others, achieving EI24% values of 55.0% and 45.7% for soybean oil and kerosene, respectively. The scanning electron microscopy (SEM) confirmed yeast growth under these conditions after 9 days. Our findings highlight the significant potential of LED light to enhance bioemulsifier production by A. pullulans LB83 from PSB. (AU)

Processo FAPESP: 16/10636-8 - Da fábrica celular à biorrefinaria integrada Biodiesel-Bioetanol: uma abordagem sistêmica aplicada a problemas complexos em micro e macroescalas
Beneficiário:Roberto de Campos Giordano
Modalidade de apoio: Auxílio à Pesquisa - Programa BIOEN - Temático
Processo FAPESP: 20/06323-0 - Produção de biossurfactantes por fermentação em estado sólido utilizando Aureobasidium pullulans com emprego de bagaço de cana-de-açúcar como substrato: do pré-tratamento da biomassa ao design de biorreatores
Beneficiário:Daylin Rubio Ribeaux
Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado