Dual-Target Additively Manufactured Electrochemical Sensor for the Multiplexed Detection of Protein A29 and DNA of Human Monkeypox Virus

Autor(es)

Silva, Luiz Ricardo G. ; Stefano, Jessica S. ; Kalinke, Cristiane ; Crapnell, Robert D. ; Brazaca, Lais C. ; Marcolino-Junior, Luiz H. ; Bergamini, Marcio F. ; Banks, Craig E. ; Janegitz, Bruno C.

• Tipo de documento: Artigo Científico
• Fonte: ACS OMEGA; v. 9, n. 30, p. 12-pg., 2024-07-17.

Resumo

Herein, we present the first 3D-printed electrochemical portable biodevice for the detection of monkeypox virus (MKPV). The electrochemical device consists of two biosensors: an immunosensor and a genosensor specifically designed for the detection of the protein A29 and a target DNA of MKPV, respectively. The electrodes were manufactured using lab-made ultraflexible conductive filaments composed of carbon black, recycled PLA from coffee pods, and castor oil as a plasticizer. The sensors created through 3D printing technology exhibited good reproducibility and repeatability of analytical responses. Furthermore, both the immunosensor and genosensor demonstrated excellent MKPV detection capabilities, with a linear range from 0.01 to 1.0 mu mol L-1 for the antigen and 0.1 to 20.0 mu mol L-1 for the DNA target. The biosensors achieved limits of detection of 2.7 and 29 nmol L-1 for the immunosensor and genosensor, respectively. Interference tests conducted with the biosensors demonstrated their selectivity for MKPV. Moreover, analyses of fortified human serum samples showed recoveries close to 100%, confirming the absence of significant matrix effects for MKPV analysis. Therefore, the 3D-printed multiplex device represents a viable and highly promising alternative for on-site, portable, and rapid point-of-care MKPV monitoring. (AU)

• Processo FAPESP: 17/21097-3 - Interações abelha-agricultura: perspectivas para a utilização sustentável
• Beneficiário: Osmar Malaspina
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOTA - Temático
• Processo FAPESP: 23/06793-4 - Desenvolvimento de sistemas eletroquímicos multiplex aplicados para detecção de biomarcadores relacionados a doenças cardiovasculares
• Beneficiário: Bruno Campos Janegitz
• Modalidade de apoio: Auxílio à Pesquisa - Regular