Comparison of biofilm models for producing artificial active white spot lesions

Autor(es)

Araujo, Erika Michele dos Santos ; Vidal, Cristina de Mattos Pimenta ; Zhu, Min ; Banas, Jeffrey A. ; Freitas, Anderson Zanardi de ; Wetter, Niklaus Ursus ; Matos, Adriana Bona

• Tipo de documento: Artigo Científico
• Fonte: Journal of Applied Oral Science; v. 32, p. 10-pg., 2024-01-01.

Resumo

Creating artificial caries-like lesions that mimic the complex changes observed in natural caries is essential for properly evaluating new strategies, dental materials, and devices designed to arrest their progression and avoid more costly and invasive treatments. Objective: This study compared three protocols for developing artificial white spot lesions (WSL) using biofilm models. Methodology: In total, 45 human enamel specimens were sterilized and allocated into three groups based on the biofilm model: Streptococcus sobrinus and Lactobacillus casei (Ss+Lc), Streptococcus sobrinus (Ss), or Streptococcus mutans (Sm). Specimens were incubated in filter-sterilized human saliva to form the acquired pellicle and then subjected to the biofilm challenge consisting of three days of incubation with bacteria (for demineralization) and one day of remineralization, which was performed once for Ss+Lc (four days total), four times for Ss (16 days total), and three times for Sm (12 days total). After WSL creation, the lesion fluorescence, depth, and chemical composition were assessed using Quantitative Lightinduced Fluorescence (QLF), Polarized Light Microscopy (PLM), and Raman Spectroscopy, respectively. Statistical analysis consisted of two-way ANOVA followed by Tukey's post hoc test (alpha=0.05). WSL created using the Ss+Lc protocol presented statistically significant higher fluorescence loss (Delta F) and integrated fluorescence (Delta Q) in comparison to the other two protocols (p<0.001). Results: In addition, Ss+Lc resulted in significantly deeper WSL (137.5 <mu>m), followed by Ss (84.1 mu m) and Sm (54.9 mu m) (p<0.001). While high mineral content was observed in sound enamel surrounding the WSL, lesions created with the Ss+Lc protocol showed the highest demineralization level and changes in the mineral content among the three protocols. Conclusion: The biofilm model using S. sobrinus and L. casei for four days was the most appropriate and simplified protocol for developing artificial active WSL with lower fluorescence, higher demineralization, and greater depth. (AU)

• Processo FAPESP: 18/19240-5 - Equipamento multiusuário (EMU) concedido no processo 2017/50332-0: sistema de análise STM-AFM Raman SNOM
• Beneficiário: Anderson Zanardi de Freitas
• Modalidade de apoio: Auxílio à Pesquisa - Programa Equipamentos Multiusuários
• Processo FAPESP: 17/50332-0 - Capacitação científica, tecnológica e em infraestrutura em radiofármacos, radiações e empreendedorismo a serviço da saúde (PDIp)
• Beneficiário: Marcelo Linardi
• Modalidade de apoio: Auxílio à Pesquisa - Programa Modernização de Institutos Estaduais de Pesquisa