| Texto completo | |
| Autor(es): Mostrar menos - |
Palombo, Paola
;
Maeda, Roberta
;
Zaniboni, Caroline Riberti
;
Engi, Sheila Antonagi
;
Yokoyama, Thais
;
Bertagna, Natalia Bonetti
;
Anesio, Augusto
;
Bianchi, Paula Cristina
;
Righi, Thamires
;
Tavares, Gabrielle Emily Boaventura
;
Souccar, Caden
;
da Silva, Fernando Bezerra Romualdo
;
Cruz, Fabio Cardoso
Número total de Autores: 13
|
| Tipo de documento: | Artigo Científico |
| Fonte: | Neuroscience Letters; v. 824, p. 10-pg., 2024-02-15. |
| Resumo | |
Alcohol Use Disorder (AUD) presents a significant and challenging public health concern, marked by a dearth of effective pharmacological treatments. Understanding the neurobiological underpinnings of AUD is of paramount importance for the development of efficacious interventions. The process of addiction entails the acquisition of associative behaviors, prominently engaging the dorsal region of the hippocampus for encoding these associative memories. Nicotinic receptor systems have been implicated in mediating the rewarding effects of ethanol, as well as memory and learning processes. In our current investigation, we delved into the role of alpha 402 nicotinic acetylcholine receptors (nAChRs) within the dorsal hippocampus in the context of ethanol-induced conditioned place preference (CPP), a robust model for scrutinizing the rewarding properties and drug-associated behaviors. To establish CPP, ethanol (2 g/kg) was administered intraperitoneally during a 8-day conditioning phase. Fos immunohistochemistry was employed to assess the involvement of discrete subregions within the dorsal hippocampus in ethanol-induced CPP. Additionally, we probed the influence of alpha 402 nAChRs on CPP via microinjections of a selective nAChR antagonist, dihydro-0-erythroidine (DHBE, at dosages of 6, 12, and 18 mu g/0.5 mu L per hemisphere) within the hippocampus. Our results unveiled that ethanol-induced CPP was associated with an increase Fos -positive cells in various subregions of the dorsal hippocampus, including CA1, CA2, CA3, and the dentate gyrus. Intrahippocampal administration of DHBE (at doses of 6 and 18 mu g/0.50 mu L per hemisphere) effectively blocked ethanol-induced CPP, while leaving locomotor activity unaffected. These findings underscore the critical involvement of the dorsal hippocampus and alpha 402 nAChRs in the acquisition of ethanol-associated learning and reward. (AU) | |
| Processo FAPESP: | 18/02889-9 - Papel dos Receptores colinérgicos nicotínicos do hipocampo dorsal na recaída da busca por etanol em camundongos. |
| Beneficiário: | Roberta Ayumi Maeda |
| Modalidade de apoio: | Bolsas no Brasil - Iniciação Científica |
| Processo FAPESP: | 13/24986-2 - Participação de pequenos grupos neurais seletivamente ativados (neuronal ensembles) na reinstalação da autoadministração de etanol induzida pelo ambiente: investigação farmacogenética, optogenética e molecular |
| Beneficiário: | Fabio Cardoso Cruz |
| Modalidade de apoio: | Auxílio à Pesquisa - Jovens Pesquisadores |