The cholinergic response is increased in isolated ileum from gastroschisis rat model

Autor(es)

Regis, Aline Cristina ^[1] ; Rojas-Moscoso, Julio Alejandro ^[2] ; Lanhellas Goncalves, Frances Lilian ^[1] ; Schmidt, Augusto Frederico ^[1] ; Monica, Fabiola Zakia ^[2] ; Antunes, Edson ^[2] ; Sbragia, Lourenco ^[3]

Afiliação do(s) autor(es)

^[1] State Univ Campinas UNICAMP, Dept Surg, Sch Med Sci, Campinas, SP - Brazil

^[2] State Univ Campinas UNICAMP, Dept Pharmacol, Campinas, SP - Brazil

^[3] USP, Div Pediat Surg, Michael R Harrison Lab Expt Fetal Surg, Dept Surg & Anat, Sch Med Ribeirao Preto, BR-14049900 Ribeirao Preto, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: PEDIATRIC SURGERY INTERNATIONAL; v. 27, n. 9, p. 1015-1019, SEP 2011.
• Citações Web of Science: 2

Resumo

Babies with gastroschisis (G) have high morbidity rate and long hospital stay due to bowel hypomotility caused by chronic exposure of the bowel to the amniotic fluid. Our aim was to evaluate the reactivity of isolated ileum in fetal rats selected for experimental gastroschisis. G was surgically created at 18.5 days of gestation (term = 22 days). Concentration-dependent curve to the muscarinic agonist methacholine (1-30 mu M) and contractions induced by electrical field stimulation (EFS, 1-16 Hz, 50 V, 1 ms) were carried out in isolated ileum of groups control (C), sham (S) and gastroschisis (G) (n = 30). Protein expression for M(3) was assessed by western blot analysis. The frequency and amplitude of spontaneous contractions were decreased in G (p < 0.001). Methacholine produced concentration-dependent contractions being the maximal response values higher in G (p < 0.01). EFS-induced frequency-dependent contractions showed 1.8 times higher in G as well as an increase of M(3) expression. The frequency and the amplitude of rhythmic contractions were reduced along with an increase in the contraction induced by mucarinic agonist and by EFS in G. These results suggest the occurrence of an adaptative supersensitivity to cholinergic response via increases in the protein expression for M(3) receptor. (AU)

• Processo FAPESP: 08/51487-9 - Avaliacao da protecao das alcas intestinais fetais utilizando hidrogel (biomaterial) e oxido nitrico no modelo experimental de gastrosquise
• Beneficiário: Lourenço Sbragia Neto
• Modalidade de apoio: Auxílio à Pesquisa - Regular