| Texto completo | |
| Autor(es): |
Al-Madboly, Lamiaa A.
;
El-Salam, Mohamed A. Abd
;
Bastos, Jairo K.
;
Aboukhatwa, Shaimaa
;
El-Morsi, Rasha M.
Número total de Autores: 5
|
| Tipo de documento: | Artigo Científico |
| Fonte: | Microbial Cell Factories; v. 23, n. 1, p. 16-pg., 2024-08-08. |
| Resumo | |
beta-lactam resistance is a significant global public health issue. Outbreaks of bacteria resistant to extended-spectrum beta-lactams and carbapenems are serious health concerns that not only complicate medical care but also impact patient outcomes. The primary objective of this work was to express and purify two soluble recombinant representative serine beta-lactamases using Escherichia coli strain as an expression host and pET101/D as a cloning vector. Furthermore, a second objective was to evaluate the potential, innovative, and safe use of galloylquinic acid (GQA) from Copaifera lucens as a potential beta-lactamase inhibitor. In the present study, bla(CTX-M-15) and bla(KPC-2) represented genes encoding for serine beta-lactamases that were cloned from parent isolates of E. coli and K. pneumoniae, respectively, and expression as well as purification were performed. Moreover, susceptibility results demonstrated that recombinant cells became resistant to all test carbapenems (MICs; 64-128 mu g/mL) and cephalosporins (MICs; 128-512 mu g/mL). The MICs of the tested beta-lactam antibiotics were determined in combination with 4 mu g/mL of GQA, clavulanic acid, or tazobactam against E. coli strains expressing CTX-M-15 or KPC-2-beta-lactamases. Interestingly, the combination with GQA resulted in an important reduction in the MIC values by 64-512-fold to the susceptible range with comparable results for other reference inhibitors. Additionally, the half-maximal inhibitory concentration of GQA was determined using nitrocefin as a beta-lactamase substrate. Data showed that the test agent was similar to tazobactam as an efficient inhibitors of the test enzymes, recording smaller IC50 values (CTX-M-15; 17.51 for tazobactam, 28.16 mu g/mL for GQA however, KPC-2; 20.91 for tazobactam, 24.76 mu g/mL for GQA) compared to clavulanic acid. Our work introduces GQA as a novel non-beta-lactam inhibitor, which interacts with the crucial residues involved in beta-lactam recognition and hydrolysis by non-covalent interactions, complementing the enzyme's active site. GQA markedly enhanced the potency of beta-lactams against carbapenemase and extended-spectrum beta-lactamase-producing strains, reducing the MICs of beta-lactams to the susceptible range. The beta-lactamase inhibitory activity of GQA makes it a promising lead molecule for the development of more potent beta-lactamase inhibitors. (AU) | |
| Processo FAPESP: | 11/13630-7 - Validação química e farmacológica de extratos e princípios ativos de espécies de Copaifera |
| Beneficiário: | Jairo Kenupp Bastos |
| Modalidade de apoio: | Auxílio à Pesquisa - Temático |