Veterinarians exposed to inhaled anesthetic present chromosome damage, apoptosis and cell cycle changes

Autor(es)

Braz, Mariana G. ; Figueiredo, Drielle B. S. ; Golim, Marjorie A. ; Grassi, Tony F. ; da Costa, Bruno R. B. ; De Martinis, Bruno S. ; Braz, Leandro G.

• Tipo de documento: Artigo Científico
• Fonte: Environmental and Molecular Mutagenesis; v. 65, n. 1-2, p. 7-pg., 2024-02-09.

Resumo

This cross-sectional study evaluated, for the first time, DNA damage, viability, and cell death of lymphocytes and cell cycle phases of mononuclear and polymorphonuclear cells in veterinarians exposed to the volatile anesthetic isoflurane. Veterinarians who were occupationally exposed to isoflurane (exposed group; n = 20) and matched-unexposed individuals (volunteers without occupational exposure; n = 20) were enrolled in the study. DNA damage was assessed in lymphocytes by micronucleus (MN) and phosphorylated histone gamma-H2AX (gamma-H2AX). Cell viability, cytotoxicity, and the cell cycle were evaluated by flow cytometry. Isoflurane was detected in urine samples by headspace gas chromatography-mass spectrometry. Compared with unexposed subjects, veterinarians occupationally exposed to isoflurane (25.7 +/- 23.7 mu g/L urine) presented statistically higher MN frequencies, lymphocytic apoptosis rates, and numbers of polymorphonuclear cells in the G0/G1 stage. Additionally, the exposed group presented statistically lower proportions of viable lymphocytes and G2/M polymorphonuclear cells. Our findings indicate that veterinarians who are frequently exposed to inhaled anesthetic exhibit chromosomal and cell damage in addition to changes in peripheral blood cell proliferation. (AU)

• Processo FAPESP: 22/10026-6 - Perfil transcricional, epigenético, metabolômico e oxidativo-inflamatório da exposição anestésica em profissionais atuantes em centro cirúrgico veterinário
• Beneficiário: Mariana Gobbo Braz
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 20/08455-0 - Impacto da exposição ocupacional aos anestésicos halogenados na instabilidade genômica e citotoxicidade em profissionais que atuam em hospital veterinário
• Beneficiário: Drielle Baptista dos Santos Figueiredo
• Modalidade de apoio: Bolsas no Brasil - Programa Capacitação - Treinamento Técnico
• Processo FAPESP: 18/20900-0 - Impacto da exposição ocupacional aos anestésicos halogenados na instabilidade genômica e citotoxicidade em profissionais que atuam em hospital veterinário
• Beneficiário: Mariana Gobbo Braz
• Modalidade de apoio: Auxílio à Pesquisa - Regular