Polyprenylated Benzophenones from Brazilian Red Propolis: Analytical Characterization and Anticancer Activity

Autor(es)

Pires, Loren M. ; Santos, Mario F. C. ; Figueiredo, Laisla R. ; Faleiros, Renata ; Badoco, Fernanda R. ; Silva, Karolinne B. ; Ambrosio, Sergio R. ; Bastos, Jairo Kenupp ; dos Santos, Raquel A. ; Veneziani, Rodrigo C. S.

• Tipo de documento: Artigo Científico
• Fonte: CHEMISTRY & BIODIVERSITY; v. N/A, p. 9-pg., 2024-10-31.

Resumo

The present work describes the extraction of a polyprenylated benzophenone-rich extract from Brazilian red propolis (ERPB), the development and validation of an RP-HPLC-UV method to characterize it, and its evaluation against breast cancer cell lines MCF-7 and MDA-MB-231, as well as the normal counterpart MCF-10 A. A mixture of gutifferone E and xanthochymol (1+2), and isolated oblongifolin B (3) were used as chemical standards for ERPB and were also evaluated. The concentrations of 1+2 and 3 corresponded to 16.68 % and 42.25 % of the total content of the extract, respectively, and the validation parameters evaluated were satisfactorily met. The cytotoxic effects of ERPB were assessed, and the obtained IC50 values were 19.58 mu g/mL (MCF-10 A), 11.56 mu g/mL (MCF-7), and 5.22 mu g/mL (MDA-MB-231). In conclusion, ERPB exhibits promising cytotoxic effects on the tested breast cell lines. However, further investigation to elucidate its potential therapeutic applications and safety profile should be conducted. (AU)

• Processo FAPESP: 17/04138-8 - Realização de estudos químicos, analíticos, biológicos, farmacológicos e tecnológicos para preenchimento das lacunas no desenvolvimento do setor de própolis brasileiro
• Beneficiário: Jairo Kenupp Bastos
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 18/05080-6 - EMU concedido no processo 2017/04138-8 para sistema de cromatografia líquida de alta pressão semipreparativo modelo LC-20AR
• Beneficiário: Sergio Ricardo Ambrosio
• Modalidade de apoio: Auxílio à Pesquisa - Programa Equipamentos Multiusuários