| Texto completo | |
| Autor(es): |
Pires, Loren M.
;
Santos, Mario F. C.
;
Figueiredo, Laisla R.
;
Faleiros, Renata
;
Badoco, Fernanda R.
;
Silva, Karolinne B.
;
Ambrosio, Sergio R.
;
Bastos, Jairo Kenupp
;
dos Santos, Raquel A.
;
Veneziani, Rodrigo C. S.
Número total de Autores: 10
|
| Tipo de documento: | Artigo Científico |
| Fonte: | CHEMISTRY & BIODIVERSITY; v. N/A, p. 9-pg., 2024-10-31. |
| Resumo | |
The present work describes the extraction of a polyprenylated benzophenone-rich extract from Brazilian red propolis (ERPB), the development and validation of an RP-HPLC-UV method to characterize it, and its evaluation against breast cancer cell lines MCF-7 and MDA-MB-231, as well as the normal counterpart MCF-10 A. A mixture of gutifferone E and xanthochymol (1+2), and isolated oblongifolin B (3) were used as chemical standards for ERPB and were also evaluated. The concentrations of 1+2 and 3 corresponded to 16.68 % and 42.25 % of the total content of the extract, respectively, and the validation parameters evaluated were satisfactorily met. The cytotoxic effects of ERPB were assessed, and the obtained IC50 values were 19.58 mu g/mL (MCF-10 A), 11.56 mu g/mL (MCF-7), and 5.22 mu g/mL (MDA-MB-231). In conclusion, ERPB exhibits promising cytotoxic effects on the tested breast cell lines. However, further investigation to elucidate its potential therapeutic applications and safety profile should be conducted. (AU) | |
| Processo FAPESP: | 17/04138-8 - Realização de estudos químicos, analíticos, biológicos, farmacológicos e tecnológicos para preenchimento das lacunas no desenvolvimento do setor de própolis brasileiro |
| Beneficiário: | Jairo Kenupp Bastos |
| Modalidade de apoio: | Auxílio à Pesquisa - Temático |
| Processo FAPESP: | 18/05080-6 - EMU concedido no processo 2017/04138-8 para sistema de cromatografia líquida de alta pressão semipreparativo modelo LC-20AR |
| Beneficiário: | Sergio Ricardo Ambrosio |
| Modalidade de apoio: | Auxílio à Pesquisa - Programa Equipamentos Multiusuários |