Detection of Hepatitis B virus subgenotype A1 in a Quilombo community from Maranhao, Brazil

Autor(es)

Alvarado-Mora, Monica V. ^{[1, 2]} ; Botelho, Livia ^{[1, 2]} ; Gomes-Gouvea, Michele S. ^{[2, 1]} ; de Souza, Vanda F. ^[3] ; Nascimento, Maria C. ^[3] ; Pannuti, Claudio S. ^[3] ; Carrilho, Flair J. ^{[1, 2]} ; Pinho, Joao R. R. ^{[1, 2]}

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Dept Gastroenterol, Sch Med, Sao Paulo - Brazil

^[2] Univ Sao Paulo, Lab Trop Gastroenterol & Hepatol, Sao Paulo Inst Trop Med, Sch Med, Sao Paulo - Brazil

^[3] Univ Sao Paulo, Virol Lab, Sao Paulo Inst Trop Med, Dept Infect & Parasit Dis, Sch Med, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: VIROLOGY JOURNAL; v. 8, AUG 25 2011.
• Citações Web of Science: 10

Resumo

Background: The Brazilian population is mainly descendant from European colonizers, Africans and Native Americans. Some Afro-descendants lived in small isolated communities since the slavery period. The epidemiological status of HBV infection in Quilombos communities from northeast of Brazil remains unknown. The aim of this study was to characterize the HBV genotypes circulating inside a Quilombo isolated community from Maranhao State, Brazil. Methods: Seventy-two samples from Frechal Quilombo community at Maranhao were collected. All serum samples were screened by enzyme-linked immunosorbent assays for the presence of hepatitis B surface antigen ( HBsAg). HBsAg positive samples were submitted to DNA extraction and a fragment of 1306 bp partially comprising HBsAg and polymerase coding regions (S/POL) was amplified by nested PCR and its nucleotide sequence was determined. Viral isolates were genotyped by phylogenetic analysis using reference sequences from each genotype obtained from GenBank (n = 320). Sequences were aligned using Muscle software and edited in the SE-AL software. Bayesian phylogenetic analyses were conducted using Markov Chain Monte Carlo (MCMC) method to obtain the MCC tree using BEAST v.1.5.3. Results: Of the 72 individuals, 9 (12.5%) were HBsAg-positive and 4 of them were successfully sequenced for the 1306 bp fragment. All these samples were genotype A1 and grouped together with other sequences reported from Brazil. Conclusions: The present study represents the first report on the HBV genotypes characterization of this community in the Maranhao state in Brazil where a high HBsAg frequency was found. In this study, we reported a high frequency of HBV infection and the exclusive presence of subgenotype A1 in an Afro-descendent community in the Maranhao State, Brazil. (AU)

• Processo FAPESP: 07/53457-7 - Estudo da variabilidade genotípica dos vírus das Hepatites B e C na Colômbia
• Beneficiário: João Renato Rebello Pinho
• Linha de fomento: Auxílio à Pesquisa - Regular
• Processo FAPESP: 08/50461-6 - Estudo da variabilidade genotípica dos vírus da Hepatite B e Hepatite C na Colômbia
• Beneficiário: Monica Viviana Alvarado Mora
• Linha de fomento: Bolsas no Brasil - Doutorado Direto