Lipidomics by Nuclear Magnetic Resonance Spectroscopy and Liquid Chromatography-High-Resolution Mass Spectrometry in Osteosarcoma: A Pilot Study

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Pontes, Joao Guilherme de Moraes ; Jadranin, Milka ; Assalin, Marcia Regina ; Escobar, Melissa Quintero ; Stanisic, Danijela ; Costa, Tassia Brena Barroso Carneiro ; Lengert, Andre van Helvoort ; Boldrini, Erica ; da Silva, Sandra Regina Morini ; Vidal, Daniel Onofre ; Liu, Leticia Huan Bacellar ; Maschietto, Mariana ; Tasic, Ljubica

• Tipo de documento: Artigo Científico
• Fonte: METABOLITES; v. 14, n. 8, p. 17-pg., 2024-08-01.

Resumo

Cancer is a complex disease that can also affect the younger population; however, it is responsible for a relatively high mortality rate of children and youth, especially in low- and middle-income countries (LMICs). Besides that, lipidomic studies in this age range are scarce. Therefore, we analyzed blood serum samples from young patients (12 to 35 years) with bone sarcoma (osteosarcoma) and compared their lipidomics to the ones from the control group of samples, named healthy control (HC group), using NMR and LC-MS techniques. Furthermore, differences in the lipidomic profiles between OS patients with and without metastasis indicate higher glycerophosphocholine (GPC) and glycerophospholipid (GPL) levels in osteosarcoma and increased cholesterol, choline, polyunsaturated fatty acids (PUFAs), and glycerols during the metastasis. These differences, detected in the peripheral blood, could be used as biomarkers for liquid biopsy. (AU)

• Processo FAPESP: 18/21047-9 - Câncer na infância - predisposição genética e mecanismos de origem
• Beneficiário: Ana Cristina Victorino Krepischi
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 23/03780-9 - Metabonômica e Metabolômica do expossoma por RMN
• Beneficiário: João Guilherme de Moraes Pontes
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 18/24069-3 - Do biomonitoramento ao reconhecimento de assinaturas do exposoma humano visando antecipar riscos para uma saúde contínua
• Beneficiário: Fernando Barbosa Júnior
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 18/06510-4 - Aplicação de Ressonância Magnética Nuclear (RMN) em metabolômica e metabonômica de doenças humanas
• Beneficiário: Ljubica Tasic
• Modalidade de apoio: Auxílio à Pesquisa - Regular