| Texto completo | |
| Autor(es): Mostrar menos - |
Higgins, Matthew
;
Kristan, Mojca
;
Collins, Emma L.
;
Messenger, Louisa A.
;
Dombrowski, Jamille G.
;
Vanheer, Leen N.
;
Nolder, Debbie
;
Drakeley, Christopher J.
;
Stone, William
;
Mahamar, Almahamoudou
;
Bousema, Teun
;
Delves, Michael
;
Bandibabone, Janvier
;
N'Do, Severin
;
Bantuzeko, Chimanuka
;
Zawadi, Bertin
;
Walker, Thomas
;
Sutherland, Colin J.
;
Marinho, Claudio R. F.
;
Cameron, Mary M.
;
Clark, Taane G.
;
Campino, Susana
Número total de Autores: 22
|
| Tipo de documento: | Artigo Científico |
| Fonte: | SCIENTIFIC REPORTS; v. 14, n. 1, p. 10-pg., 2024-08-30. |
| Resumo | |
Robust diagnostic tools and surveillance are crucial for malaria control and elimination efforts. Malaria caused by neglected Plasmodium parasites is often underestimated due to the lack of rapid diagnostic tools that can accurately detect these species. While nucleic-acid amplification technologies stand out as the most sensitive methods for detecting and confirming Plasmodium species, their implementation in resource-constrained settings poses significant challenges. Here, we present a Pan Plasmodium recombinase polymerase amplification lateral flow (RPA-LF) assay, capable of detecting all six human infecting Plasmodium species in low resource settings. The Pan Plasmodium RPA-LF assay successfully detected low density clinical infections with a preliminary limit of detection between 10-100 fg/mu l for P. falciparum. When combined with crude nucleic acid extraction, the assay can serve as a point-of-need tool for molecular xenomonitoring. This utility was demonstrated by screening laboratory-reared Anopheles stephensi mosquitoes fed with Plasmodium-infected blood, as well as field samples of An. funestus s.l. and An. gambiae s.l. collected from central Africa. Overall, our proof-of-concept Pan Plasmodium diagnostic tool has the potential to be applied for clinical and xenomonitoring field surveillance, and after further evaluation, could become an essential tool to assist malaria control and elimination. (AU) | |
| Processo FAPESP: | 22/02771-3 - Análise genômica do Plasmodium vivax e Plasmodium falciparum na malária placentária |
| Beneficiário: | Jamille Gregório Dombrowski |
| Modalidade de apoio: | Bolsas no Exterior - Estágio de Pesquisa - Pós-Doutorado |
| Processo FAPESP: | 19/12068-5 - Identificação de biomarcadores preditivos de disfunção placentária na Malária |
| Beneficiário: | Jamille Gregório Dombrowski |
| Modalidade de apoio: | Bolsas no Brasil - Pós-Doutorado |
| Processo FAPESP: | 22/13150-0 - Inflamação induzida por Malária: uma nova via para doenças cardiovasculares |
| Beneficiário: | Cláudio Romero Farias Marinho |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |