| Texto completo | |
| Autor(es): Mostrar menos - |
Meyer, Maria Davoli
;
Coelho, Rogerio Meneses Ibiapina
;
Rangel-Coelho, Joao Pedro
;
Costa, Bruna Carolina
;
Teixeira, Lucas Novaes
;
Martinez, Elizabeth Ferreira
;
Casarin, Renato Correa Viana
;
Santamaria, Mauro Pedrine
;
Franca, Fabiana Mantovani Gomes
;
Nociti-Jr, Francisco Humberto
;
Lisboa-Filho, Paulo Noronha
;
Kantovitz, Kamila Rosamilia
Número total de Autores: 12
|
| Tipo de documento: | Artigo Científico |
| Fonte: | COLLOIDS AND SURFACES B-BIOINTERFACES; v. 246, p. 10-pg., 2025-02-01. |
| Resumo | |
The objective was to address the repercussion of adding titanium dioxide nanotubes (TiO2-nt) into high-viscosity conventional glass ionomer cement (GIC) on the biological properties of pre-odontoblastic cells (MDPC-23) challenged by lipopolysaccharides (LPS - 2 mu g/mL). TiO2-nt was added to Ketac Molar EasyMix at 3, 5, 7 %, whereas unblended GIC served as control. Analyses included proliferation (n=6; 24, 48, 72 h), metabolism (MTT; n=6; 24, 48, 72 h); morphology laser microscopy (n=3; 24, 48, 72 h); proteome assessments IL-1 beta, IL-6, IL-10, VEGF, TNF-alpha (n=3; 12, 18 h); mRNA levels (RT-PCR) of Il-1 beta, Il-6, Il-10, VEGF, TNF-alpha (n=3; 12, 18 h) and DSPP (n=3; 24, 72, 120 h). Data analysis included Shapiro-Wilk, Levene, and generalized linear models (alpha=0.05). Results demonstrated that cell proliferation increased over time for all groups, and was not impacted by TiO2-nt (p>0.05). GIC groups displayed lower MTT values compared to cells cultured without GIC discs (p=0.019); disregarding the presence of TiO2. Remarkably, TiO2-nt reversed the effect of GIC, reducing the levels of selected biomarkers. LPS treatment modified the expression of the immune-inflammatory markers by MDPC-23 cells (p<0.0001). Morphological analysis did not reveal distinctions for any of the studied. TiO2-nt modulated immune-inflammatory and dentin marker expression by MDPC-23 cells cultured on conventional GIC discs, and did not affect cell morphology/viability, regardless LPS exposure. In conclusion, TiO2-nt may become a reliable clinical strategy to encourage pulp tissue repair. (AU) | |
| Processo FAPESP: | 20/15865-0 - Controle da expressão de marcadores imuno-inflamatórios em cimento de ionômero de vidro convencional incorporado de nanotubos de titânio sobre células odontoblásticas |
| Beneficiário: | Maria Davoli Meyer |
| Modalidade de apoio: | Bolsas no Brasil - Iniciação Científica |
| Processo FAPESP: | 19/14078-8 - Avaliação do efeito da incorporação de nanotubos de dióxido de titânio ao cimento de ionômero de vidro convencional sobre suas propriedades biológicas |
| Beneficiário: | Kamila Rosamilia Kantovitz |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |