The Antibacterial Potential of Brazilian Red Propolis against the Formation and Eradication of Biofilm of Helicobacter pylori

Autor(es)

Santiago, Mariana B. ; Tanimoto, Matheus H. ; Ambrosio, Maria Anita L. V. ; Veneziani, Rodrigo Cassio S. ; Bastos, Jairo K. ; Sabino-Silva, Robinson ; Martins, Carlos Henrique G.

• Tipo de documento: Artigo Científico
• Fonte: ANTIBIOTICS-BASEL; v. 13, n. 8, p. 14-pg., 2024-08-01.

Resumo

Helicobacter pylori is associated with gastrointestinal diseases, and its treatment is challenging due to antibiotic-resistant strains, necessitating alternative therapies. Brazilian red propolis (BRP), known for its diverse bioactive compounds with pharmaceutical properties, was investigated for its anti-H. pylori activity, focusing on biofilm formation inhibition and eradication. BRP was tested against H. pylori (ATCC 43526) using several assays: time-kill, nucleotide leakage, biofilm formation inhibition (determining the minimum inhibitory concentration of biofilm of 50%-MICB50, and cell viability), and biofilm eradication (determining the minimum eradication concentration of biofilm of 99.9%-MBEC). Standardization of H. pylori biofilm formation was also conducted. In the time-kill assay, BRP at 50 mu g/mL eliminated all H. pylori cells after 24 h. The nucleotide leakage assay showed no significant differences between control groups and BRP-treated groups at 25 mu g/mL and 50 mu g/mL. H. pylori formed biofilms in vitro at 10(9) CFU/mL after 72 h. The MICB50 of BRP was 15.6 mu g/mL, and at 500, 1000, and 2000 mu g/mL, BRP eradicated all bacterial cells. The MBEC was 2000 mu g/mL. These findings suggest that BRP has promising anti-H. pylori activity, effectively inhibiting and eradicating biofilms. Further studies are necessary to elucidate BRP's mechanisms of action against H. pylori. (AU)

• Processo FAPESP: 17/04138-8 - Realização de estudos químicos, analíticos, biológicos, farmacológicos e tecnológicos para preenchimento das lacunas no desenvolvimento do setor de própolis brasileiro
• Beneficiário: Jairo Kenupp Bastos
• Modalidade de apoio: Auxílio à Pesquisa - Temático