| Texto completo | |
| Autor(es): |
Santiago, Mariana B.
;
Tanimoto, Matheus H.
;
Ambrosio, Maria Anita L. V.
;
Veneziani, Rodrigo Cassio S.
;
Bastos, Jairo K.
;
Sabino-Silva, Robinson
;
Martins, Carlos Henrique G.
Número total de Autores: 7
|
| Tipo de documento: | Artigo Científico |
| Fonte: | ANTIBIOTICS-BASEL; v. 13, n. 8, p. 14-pg., 2024-08-01. |
| Resumo | |
Helicobacter pylori is associated with gastrointestinal diseases, and its treatment is challenging due to antibiotic-resistant strains, necessitating alternative therapies. Brazilian red propolis (BRP), known for its diverse bioactive compounds with pharmaceutical properties, was investigated for its anti-H. pylori activity, focusing on biofilm formation inhibition and eradication. BRP was tested against H. pylori (ATCC 43526) using several assays: time-kill, nucleotide leakage, biofilm formation inhibition (determining the minimum inhibitory concentration of biofilm of 50%-MICB50, and cell viability), and biofilm eradication (determining the minimum eradication concentration of biofilm of 99.9%-MBEC). Standardization of H. pylori biofilm formation was also conducted. In the time-kill assay, BRP at 50 mu g/mL eliminated all H. pylori cells after 24 h. The nucleotide leakage assay showed no significant differences between control groups and BRP-treated groups at 25 mu g/mL and 50 mu g/mL. H. pylori formed biofilms in vitro at 10(9) CFU/mL after 72 h. The MICB50 of BRP was 15.6 mu g/mL, and at 500, 1000, and 2000 mu g/mL, BRP eradicated all bacterial cells. The MBEC was 2000 mu g/mL. These findings suggest that BRP has promising anti-H. pylori activity, effectively inhibiting and eradicating biofilms. Further studies are necessary to elucidate BRP's mechanisms of action against H. pylori. (AU) | |
| Processo FAPESP: | 17/04138-8 - Realização de estudos químicos, analíticos, biológicos, farmacológicos e tecnológicos para preenchimento das lacunas no desenvolvimento do setor de própolis brasileiro |
| Beneficiário: | Jairo Kenupp Bastos |
| Modalidade de apoio: | Auxílio à Pesquisa - Temático |