Tailoring the structure and self-activated photoluminescence of carbonated amorphous calcium phosphate nanoparticles for bioimaging applications

Autor(es)

Machado, Thales R. ; Zanardo, Carlos E. ; Vilela, Raquel R. C. ; Miranda, Renata R. ; Moreno, Natalia S. ; Leite, Celisnolia M. ; Longo, Elson ; Zucolotto, Valtencir

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF MATERIALS CHEMISTRY B; v. 12, n. 20, p. 17-pg., 2024-04-18.

Resumo

Self-activated luminescent calcium phosphate (CaP) nanoparticles, including hydroxyapatite (HA) and amorphous calcium phosphate (ACP), are promising for bioimaging and theragnostic applications in nanomedicine, eliminating the need for activator ions or fluorophores. In this study, we developed luminescent and stable citrate-functionalized carbonated ACP nanoparticles for bioimaging purposes. Our findings revealed that both the CO32- content and the posterior heating step at 400 degrees C significantly influenced the composition and the structural ordering of the chemically precipitated ACP nanoparticles, impacting the intensity, broadness, and position of the defect-related photoluminescence (PL) emission band. The heat-treated samples also exhibited excitation-dependent PL under excitation wavelengths typically used in bioimaging (lambda exc = 405, 488, 561, and 640 nm). Citrate functionalization improved the PL intensity of the nanoparticles by inhibiting non-radiative deactivation mechanisms in solution. Additionally, it resulted in an increased colloidal stability and reduced aggregation, high stability of the metastable amorphous phase and the PL emission for at least 96 h in water and supplemented culture medium. MTT assay of HepaRG cells, incubated for 24 and 48 h with the nanoparticles in concentrations ranging from 10 to 320 mu g mL-1, evidenced their high biocompatibility. Internalization studies using the nanoparticles self-activated luminescence showed that cellular uptake of the nanoparticles is both time (4-24 h) and concentration (160-320 mu g mL-1) dependent. Experiments using confocal laser scanning microscopy allowed the successful imaging of the nanoparticles inside cells via their intrinsic PL after 4 h of incubation. Our results highlight the potential use of citrate-functionalized carbonated ACP nanoparticles for use in internalization assays and bioimaging procedures. The optimization of carbonates concentration, a posterior heat treatment step, and citrate functionalization yield stable self-activated luminescent amorphous calcium phosphate nanoparticles for bioimaging applications. (AU)

• Processo FAPESP: 21/03559-5 - Desenvolvimento de nanopartículas luminescentes de fosfato de cálcio amorfo para aplicação em Nanomedicina
• Beneficiário: Carlos Eduardo Zanardo
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 20/14417-4 - Novos nanossistemas biomiméticos a base de ortofosfatos de cálcio recobertos com membranas celulares para aplicação em bioimagem e liberação controlada de antineoplásicos
• Beneficiário: Thales Rafael Machado
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado