Whole chromosome painting reveals independent origin of sex chromosomes in closely related forms of a fish species

Autor(es)

Cioffi, Marcelo de Bello ^[1] ; Sanchez, Antonio ^[2] ; Alberto Marchal, Juan ^[2] ; Kosyakova, Nadezda ^[3] ; Liehr, Thomas ^[3] ; Trifonov, Vladimir ^[4] ; Carlos Bertollo, Luiz Antonio ^[1]

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Carlos, Dept Genet & Evolucao, Sao Carlos, SP - Brazil

^[2] Univ Jaen, Dept Biol Expt, Fac Ciencias Expt, Jaen - Spain

^[3] Jena Univ Hosp, Inst Human Genet, D-07743 Jena - Germany

^[4] SB RAS, Inst Chem Biol & Fundamental Med, Novosibirsk - Russia

• Tipo de documento: Artigo Científico
• Fonte: Genetica; v. 139, n. 8, p. 1065-1072, AUG 2011.
• Citações Web of Science: 17
• Assunto(s): Cromossomos sexuais Microdissecção Cromossomo X Cromossomo Y

Resumo

The wolf fish Hoplias malabaricus includes well differentiated sex systems (XY and X(1)X(2)Y in karyomorphs B and D, respectively), a nascent XY pair (karyomorph C) and not recognized sex chromosomes (karyomorph A). We performed the evolutionary analysis of these sex chromosomes, using two X chromosome-specific probes derived by microdissection from the XY and X(1)X(2)Y sex systems. A putative-sex pair in karyomorph A was identified, from which the differentiated XY system was evolved, as well as the clearly evolutionary relationship between the nascent XY system and the origin of the multiple X(1)X(2)Y chromosomes. The lack of recognizable signals on the sex chromosomes after the reciprocal cross-FISH experiments highlighted that they evolved independently from non-homologous autosomal pairs. It is noteworthy that these distinct pathways occur inside the same nominal species, thus exposing the high plasticity of sex chromosome evolution in lower vertebrates. Possible mechanisms underlying this sex determination liability are also discussed. (AU)

• Processo FAPESP: 09/14881-3 - Evolução cromossômica na família Erythrinidae: mapeamento citogenético de DNAs repetitivos e microdissecção de cromossomos sexuais
• Beneficiário: Marcelo de Bello Cioffi
• Modalidade de apoio: Bolsas no Brasil - Doutorado