| Texto completo | |
| Autor(es): |
Jacinavicius, Fernanda Rios
;
Campos, Thaissa Giovanna Valverde
;
Passos, Larissa Souza
;
Pinto, Ernani
;
Geraldes, Vanessa
Número total de Autores: 5
|
| Tipo de documento: | Artigo Científico |
| Fonte: | Toxicon; v. 234, p. 6-pg., 2023-09-08. |
| Resumo | |
Cyanobacteria can form harmful blooms in specific environmental conditions due to certain species producing toxic metabolites known as cyanotoxins. These toxins pose significant risks to public health and the environment, making it critical to identify and quantify them in food and water sources to avoid contamination. However, current screening methods only focus on a single class of cyanotoxins, limiting their effectiveness. Thus, fast and sensitive liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) method was developed to analyze eighteen cyanotoxins simultaneously. A simplified extraction procedure using lyophilized samples of cyanobacterial biomass was also used, eliminating the need for traditional solid-phase extraction methods. This method uses multiple reaction monitoring and allows accurate determination and quantification of eighteen cyanotoxins, including anatoxin-a, homoanatoxin-a, cylindrospermopsin, deoxy-cylindrospermopsin, nodularin, guanitoxin, seven microcystins (RR, [D-Asp3] RR, LA, LR, LY, LW, and YR), and five saxitoxins (gonyautoxins -GTX-1&4, GTX-2&3, GTX-5), decarbamoylgonyautoxin (dcGTX-2&3), and N-Sulfocarbamoylgonyautoxin (C1&C2), all in a short acquisition time of 8 min. Therefore, this method provides a simple and efficient approach to identify and quantify harmful compounds produced by cyanobacteria. Hence, this represents the first method to detecting guanitoxin among cyanotoxins. By expanding the range of toxins analyzed, this method can help ensure high-quality food and drinking water and protect recreational users from exposure to cyanotoxins. (AU) | |
| Processo FAPESP: | 13/07914-8 - FoRC - Centro de Pesquisa em Alimentos |
| Beneficiário: | Bernadette Dora Gombossy de Melo Franco |
| Modalidade de apoio: | Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs |
| Processo FAPESP: | 21/00149-0 - Tratamento e fracionamento seletivo de cianobactérias em florações para produção de insumos de valor agregado |
| Beneficiário: | Ernani Pinto Junior |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |