Insights into the global genomic features of Salmonella enterica serovar Gallinarum biovars Gallinarum and Pullorum

Autor(es)

Campos, Isabela C. ; Vilela, Felipe Pinheiro ; Saraiva, Mauro de M. S. ; Junior, Angelo Berchieri ; Falcao, Juliana Pfrimer

• Tipo de documento: Artigo Científico
• Fonte: Journal of Applied Microbiology; v. 135, n. 9, p. 11-pg., 2024-09-02.

Resumo

Aims Characterize global genomic features of 86 genomes of Salmonella Gallinarum (SG) and Pullorum (SP), which are important pathogens causing systemic infections in poultry.Methods and results All genomes harbored efflux pump encoding gene mdsA and gold tolerance genes golS and golT. Aminoglycoside (aac(6 ')-Ib, aadA5, aph(6)-Id, aph(3 '')-Ib, ant(2 '')-Ia), beta-lactam (blaTEM-1, blaTEM-135), efflux pump (mdsB), fosfomycin (fosA3), sulfonamide (sul1, sul2), tetracycline [tet(A)], trimethoprim (dfrA17), acid (asr), and disinfectant (qacEdelta1) resistance genes, gyrA, gyrB, and parC quinolone resistance point mutations, and mercury tolerance genes (mer) were found in different frequencies. Additionally, 310 virulence genes, pathogenicity islands (including SPI-1, 2, 3, 4, 5, 6, 9, 10, 12, 13, and 14), plasmids [IncFII(S), ColpVC, IncX1, IncN, IncX2, and IncC], and prophages (Fels-2, ST104, 500465-1, pro483, Gifsy-2, 103 203_sal5, Fels-1, RE-2010, vB_SenS-Ent2, and L-413C) were detected. MLST showed biovar-specific sequence types, and core genome MLST showed country-specific and global-related clusters.Conclusion SG and SP global strains carry many virulence factors and important antimicrobial resistance genes. The diverse plasmids and prophages suggest genetic variability. MLST and cgMLST differentiated biovars and showed profiles occurring locally or worldwide. (AU)

• Processo FAPESP: 21/07365-0 - Avaliação genômica, transcriptômica e fenotípica de aspectos da virulência de linhagens de Salmonella Infantis isoladas de fontes diversas no Brasil
• Beneficiário: Felipe Pinheiro Vilela
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 22/07013-0 - Análises do genoma, transcriptoma, construção de mutantes e caracterização fenotípica de linhagens de Campylobacter coli isoladas de diversas fontes durante 25 anos no Brasil
• Beneficiário: Juliana Pfrimer Falcão
• Modalidade de apoio: Auxílio à Pesquisa - Regular