| Texto completo | |
| Autor(es): Mostrar menos - |
Pinoti, Vitor F.
;
Ferreira, Pedro B.
;
Strini, Edward J.
;
Lubini, Greice
;
Thome, Vanessa
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Cruz, Joelma O.
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Aziani, Rodrigo
;
Quiapim, Andrea C.
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Pinto, Andressa P. A.
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Araujo, Ana Paula U.
;
De Paoli, Henrique C.
;
Pranchevicius, Maria Cristina S.
;
Goldman, Maria Helena S.
Número total de Autores: 13
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| Tipo de documento: | Artigo Científico |
| Fonte: | Journal of Experimental Botany; v. 75, n. 20, p. 19-pg., 2024-09-06. |
| Resumo | |
Successful plant reproduction depends on the adequate development of floral organs controlled by cell proliferation and other processes. The Stigma/style cell-cycle inhibitor 1 (SCI1) gene regulates cell proliferation and affects the final size of the female reproductive organ. To unravel the molecular mechanism exerted by Nicotiana tabacum SCI1 in cell proliferation control, we searched for its interaction partners through semi-in vivo pull-down experiments, uncovering a cyclin-dependent kinase, NtCDKG;2. Bimolecular fluorescence complementation and co-localization experiments showed that SCI1 interacts with NtCDKG;2 and its cognate NtCyclin L in nucleoli and splicing speckles. The screening of a yeast two-hybrid cDNA library using SCI1 as bait revealed a novel DEAD-box RNA helicase (NtRH35). Interaction between the NtCDKG;2-NtCyclin L complex and NtRH35 is also shown. Subcellular localization experiments showed that SCI1, NtRH35, and the NtCDKG;2-NtCyclin L complex associate with each other within splicing speckles. The yeast two-hybrid screening of NtCDKG;2 and NtRH35 identified the conserved spliceosome components U2a', NF-kappa B activating protein (NKAP), and CACTIN. This work presents SCI1 and its interactors, the NtCDKG;2-NtCyclin L complex and NtRH35, as new spliceosome-associated proteins. Our findings reveal a network of interactions and indicate that SCI1 may regulate cell proliferation through the splicing process, providing new insights into the intricate molecular pathways governing plant development. (AU) | |
| Processo FAPESP: | 19/24774-1 - A importância do gene SCI1 no meristema floral, sua regulação transcricional e conservação evolutiva |
| Beneficiário: | Maria Helena de Souza Goldman |
| Modalidade de apoio: | Auxílio à Pesquisa - Temático |
| Processo FAPESP: | 12/50562-2 - Estudo de SCI1 e suas interações proteína-proteína, que resultam na inibição do ciclo celular |
| Beneficiário: | Maria Helena de Souza Goldman |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 16/20486-3 - Decifrando SCI1, sua expressão durante o desenvolvimento floral e os mecanismos de ação para o controle da proliferação celular |
| Beneficiário: | Maria Helena de Souza Goldman |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |