Discovery, structural characterization, and functional insights into a novel apiosidase from the GH140 family, isolated from a lignocellulolytic-enriched mangrove microbial community

Autor(es)

Liberato, Marcelo Vizona ; Paixao, Douglas Antonio Alvaredo ; Tomazetto, Geizecler ; Ndeh, Didier ; Bolam, David N. ; Squina, Fabio Marcio

• Tipo de documento: Artigo Científico
• Fonte: Biotechnology Letters; v. 46, n. 2, p. 11-pg., 2024-01-27.

Resumo

ObjectivesApiosidases are enzymes that cleave the glycosidic bond between the monosaccharides linked to apiose, a branched chain furanose found in the cell walls of vascular plants and aquatic monocots. There is biotechnological interest in this enzyme group because apiose is the flavor-active compound of grapes, fruit juice, and wine, and the monosaccharide is found to be a plant secondary metabolite with pharmaceutical properties. However, functional and structural studies of this enzyme family are scarce. Recently, a glycoside hydrolase family member GH140 was isolated from Bacteroides thetaiotaomicron and identified as an endo-apiosidase.ResultsThe structural characterization and functional identification of a second GH140 family enzyme, termed MmApi, discovered through mangrove soil metagenomic approach, are described. Among the various substrates tested, MmApi exhibited activity on an apiose-containing oligosaccharide derived from the pectic polysaccharide rhamnogalacturonan-II. While the crystallographic model of MmApi was similar to the endo-apiosidase from Bacteroides thetaiotaomicron, differences in the shape of the binding sites indicated that MmApi could cleave apioses within oligosaccharides of different compositions.ConclusionThis enzyme represents a novel tool for researchers interested in studying the physiology and structure of plant cell walls and developing biocatalytic strategies for drug and flavor production. (AU)

• Processo FAPESP: 17/12597-2 - Estrutura e função de três hidrolases de glicosídeos multimodulares relacionadas a quebra de celulose, hemicelulose e pectina, e a influência dos módulos acessórios na ativiade catalítica.
• Beneficiário: Marcelo Vizoná Liberato
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Pós-Doutorado
• Processo FAPESP: 14/04105-4 - Caracterização estrutural e funcional de novas celulases com foco na relação entre domínios catalíticos e CBMs
• Beneficiário: Marcelo Vizoná Liberato
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 20/05784-3 - EMU concedido no processo 15/50590-4: sistema cromatográfico e detectores para análise de açúcares e monolignois de lignocelulose
• Beneficiário: Fábio Márcio Squina
• Modalidade de apoio: Auxílio à Pesquisa - Programa Equipamentos Multiusuários
• Processo FAPESP: 22/08958-8 - Desenvolvimento de microrganismos e enzimas termofílicas envolvidas na degradação e reaproveitamento de plásticos fósseis
• Beneficiário: Fábio Márcio Squina
• Modalidade de apoio: Auxílio à Pesquisa - Regular