Biofilm Dissolution and Cleaning Ability of Different Irrigant Solutions on Intraorally Infected Dentin

Autor(es)

del Carpio-Perochena, Aldo E. ; Bramante, Clovis M. ; Duarte, Marco A. H. ; Cavenago, Bruno Cavalini ; Villas-Boas, Marcelo H. ; Graeff, Marcia S. ^[1] ; Bernardineli, Norberti ; de Andrade, Flaviana Bombarda ; Ordinola-Zapata, Ronald ^[2]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Integrated Res Ctr CIP, Bauru Dent Sch Bauru, BR-17012901 Sao Paulo - Brazil

^[2] Univ Sao Paulo, Fac Odontol Bauru, Dept Endodont, Bauru Dent Sch Bauru, BR-17012901 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF ENDODONTICS; v. 37, n. 8, p. 1134-1138, AUG 2011.
• Citações Web of Science: 40

Resumo

Introduction: The aim of this study was to evaluate the biofilm dissolution and cleaning ability of different irrigant solutions on intraorally infected dentin. Methods: One hundred twenty bovine dentin specimens were infected intraorally by using a removable orthodontic device. Thirty samples were used for each irrigant solution: 2% chlorhexidine and 1%, 2.5%, and 5.25% sodium hypochlorite (NaOCl). The solutions were used for 5, 15, and 30 minutes and at 2 experimental volumes, 500 mu L and 1 mL. The samples were stained by using acridine orange dye before and after the experiments and evaluated by using a confocal microscope. The percentage of biofilm, isolated cells, and noncolonized dentin was measured by using a grid system. Differences in the reduction or increase of the studied parameters were assessed by using nonparametric methods (P < .05). Results: The higher values of biofilm dissolution and noncolonized dentin were found in the 30-minute NaOCl group and in the 5-minute and 15-minute groups of 5.25% NaOCL. The use of 2% chlorhexidine solution did not improve the biofilm dissolution or increase the cleaning of the dentin in comparison with the NaOCl solutions (P < .05). Conclusions: Two percent chlorhexidine does not dissolve the biofilms. Thirty minutes of NaOCl are necessary to have higher values of biofilm dissolution and to increase the cleaning of the dentin independently of the concentration in comparison with the 5-minute and 15-minute contact times. (J Endod 2011;37:1134-1138) (AU)

• Processo FAPESP: 09/03217-5 - Capacidade de dissolução do Hipoclorito de Sódio e da Clorexidina sobre Biofilme oral formado "in situ"
• Beneficiário: Aldo Enrique Del Carpio Perochena
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 10/16002-4 - Efeito das soluções químicas e dos meios físicos utilizados na irrigação endodôntica na dentina infectada por biofilmes orais
• Beneficiário: Ronald Ordinola Zapata
• Modalidade de apoio: Bolsas no Brasil - Doutorado