| Full text | |
| Author(s): |
del Carpio-Perochena, Aldo E.
;
Bramante, Clovis M.
;
Duarte, Marco A. H.
;
Cavenago, Bruno Cavalini
;
Villas-Boas, Marcelo H.
;
Graeff, Marcia S.
[1]
;
Bernardineli, Norberti
;
de Andrade, Flaviana Bombarda
;
Ordinola-Zapata, Ronald
[2]
Total Authors: 9
|
| Affiliation: | [1] Univ Sao Paulo, Integrated Res Ctr CIP, Bauru Dent Sch Bauru, BR-17012901 Sao Paulo - Brazil
[2] Univ Sao Paulo, Fac Odontol Bauru, Dept Endodont, Bauru Dent Sch Bauru, BR-17012901 Sao Paulo - Brazil
Total Affiliations: 2
|
| Document type: | Journal article |
| Source: | JOURNAL OF ENDODONTICS; v. 37, n. 8, p. 1134-1138, AUG 2011. |
| Web of Science Citations: | 40 |
| Abstract | |
Introduction: The aim of this study was to evaluate the biofilm dissolution and cleaning ability of different irrigant solutions on intraorally infected dentin. Methods: One hundred twenty bovine dentin specimens were infected intraorally by using a removable orthodontic device. Thirty samples were used for each irrigant solution: 2% chlorhexidine and 1%, 2.5%, and 5.25% sodium hypochlorite (NaOCl). The solutions were used for 5, 15, and 30 minutes and at 2 experimental volumes, 500 mu L and 1 mL. The samples were stained by using acridine orange dye before and after the experiments and evaluated by using a confocal microscope. The percentage of biofilm, isolated cells, and noncolonized dentin was measured by using a grid system. Differences in the reduction or increase of the studied parameters were assessed by using nonparametric methods (P < .05). Results: The higher values of biofilm dissolution and noncolonized dentin were found in the 30-minute NaOCl group and in the 5-minute and 15-minute groups of 5.25% NaOCL. The use of 2% chlorhexidine solution did not improve the biofilm dissolution or increase the cleaning of the dentin in comparison with the NaOCl solutions (P < .05). Conclusions: Two percent chlorhexidine does not dissolve the biofilms. Thirty minutes of NaOCl are necessary to have higher values of biofilm dissolution and to increase the cleaning of the dentin independently of the concentration in comparison with the 5-minute and 15-minute contact times. (J Endod 2011;37:1134-1138) (AU) | |
| FAPESP's process: | 09/03217-5 - Biofilm dissolution and cleaning ability of different irrigant solutions on intraorally infected dentin |
| Grantee: | Aldo Enrique Del Carpio Perochena |
| Support Opportunities: | Scholarships in Brazil - Master |
| FAPESP's process: | 10/16002-4 - On the chemichal and physical endodontic methods to decontaminate intraorally infected dentin |
| Grantee: | Ronald Ordinola Zapata |
| Support Opportunities: | Scholarships in Brazil - Doctorate |