Autor(es)

Guilger-Casagrande, Mariana ; Migliorini, Bianca Bertolini ; Germano-Costa, Tais ; Bilesky-Jose, Natalia ; Harada, Liliam Katsue ; Campos, Estefania Vangelie Ramos ; Goncalves, Kelly Cristina ; Polanczyk, Ricardo Antonio ; Fraceto, Leonardo Fernandes ; Lima, Renata

• Tipo de documento: Artigo Científico
• Fonte: Pest Management Science; v. 80, n. 3, p. 13-pg., 2023-11-20.

Resumo

BACKGROUND: Biogenic metallic and oxide metal nanoparticles have potential as alternatives for several current problems in agriculture, such as the control of caterpillars which cause huge losses in the production of important crops. In the present study, capped and uncapped silver, iron oxide and titanium dioxide nanoparticles were synthesized from the filtrate of Beauveria bassiana and evaluated in regard to physico-chemical characteristics, capping composition, cytotoxicity, genotoxicity and biological activity on Helicoverpa armigera and Spodoptera frugiperda caterpillars.RESULTS: A difference in the physico-chemical parameters of the capped and uncapped nanoparticles was observed, with larger aggregation and lower stability of the uncapped. In regard to the study of the capping, the presence of functional groups of biomolecules as well as the activity of B. bassiana hydrolytic enzymes were observed. Cytotoxic effects on the tested cell lines were not observed and DNA damage levels increased with more intense effects of the uncapped nanoparticles. In regard to the biological activity against Noctuidae pests, the uncapped and capped iron oxide, and uncapped titanium dioxide nanoparticles occasioned higher mortality (76%, 60% and 51%, respectively) but no differences in LC50 were recorded. Moreover, sublethal effects were reported on Helicoverpa armigera whereas Spodoptera frugiperda showed low susceptibility to the nanoparticles.CONCLUSION: The results demonstrate that biogenic metallic and oxide metal nanoparticles might show promising effects for the control of caterpillars which cause damage on important agricultural crops. Further investigations are necessary to understand the mechanisms of action and optimize the biological activity of these new nanomaterials.(c) 2023 Society of Chemical Industry. (AU)

• Processo FAPESP: 17/21004-5 - Agricultura, micro/nanotecnologia e ambiente: da avaliação dos mecanismos de ação a estudos de transporte e toxicidade
• Beneficiário: Leonardo Fernandes Fraceto
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 21/12643-0 - Associação de nanopartículas biogênicas de sílica obtidas a partir da casca de arroz como estratégia de aumento de biodisponibilidade de Beauveria bassiana para controle de pragas
• Beneficiário: Liliam Katsue Harada Rocha
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 17/20932-6 - Síntese verde e avaliação de nanopartículas de ferro baseadas em Trichoderma harzianum visando aplicação na agricultura
• Beneficiário: Natalia Bilesky José
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 17/25702-9 - Avaliação in vitro da toxicidade de sistemas carreadores para o desenvolvimento de repelentes de longa duração em modelos 2D, co-cultura e 3D
• Beneficiário: Tais Germano da Costa
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 20/05816-2 - Estudo do recobrimento (capping) de nanopartículas biogênicas e sua correlação com toxicidade e atividade no controle de fitopatógenos
• Beneficiário: Mariana Guilger Casagrande
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado