| Texto completo | |
| Autor(es): |
Laureano, Ana Flavia Santarine
;
Vigato, Aryane Alves
;
Puzer, Luciano
;
de Araujo, Daniele Ribeiro
Número total de Autores: 4
|
| Tipo de documento: | Artigo Científico |
| Fonte: | ACS APPLIED BIO MATERIALS; v. N/A, p. 11-pg., 2024-06-18. |
| Resumo | |
Human tissue kallikrein-related peptidase 7 (KLK7) is a serine protease implicated in the physiology of skin desquamation, and its uncontrolled activity can lead to chronic diseases such as psoriasis, atopic dermatitis, and Netherton syndrome. For this reason, kallikrein 7 has been identified as a potential therapeutic target. This work aimed to evaluate Pluronic (PL) hydrogels as topical carriers of four specific scFv-Fc antibodies to inhibit KLK7. The hydrogels comprised PL F127 (30% w/v) alone and a binary F127/P123 (28-2% w/v) system. Each formulation was loaded with 1 mu g/mL of each antibody and characterized by physicochemical and pharmaceutical techniques, considering antibody-micelle interactions and hydrogel behavior as smart delivery systems. Results showed that the antibodies were successfully loaded into the PL-based systems, and the sol-gel transition temperature was shifted to high values after the P123 addition. The antibodies released from the gels preserved their rheological properties (G ' > G '', 35- to 41-fold) and inhibitory activity against KLK7, even after 24 h. This work presented potential agents targeting KLK7 that may provide strategies for treating skin abnormalities. (AU) | |
| Processo FAPESP: | 19/20303-4 - Organogéis termosensíveis como estratégias para o tratamento de processos inflamatórios: da estrutura supramolecular à avaliação farmacológica |
| Beneficiário: | Daniele Ribeiro de Araujo |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 19/14773-8 - Organogéis lecitina-poloxamer como carreadores de fármacos para aplicação tópica: desenvolvimento, perfil de permeação e avaliação estrutural do estrato córneo |
| Beneficiário: | Aryane Alves Vigato |
| Modalidade de apoio: | Bolsas no Brasil - Doutorado Direto |
| Processo FAPESP: | 16/21441-3 - Desenvolvimento de Inibidores para as Calicreínas Tecidas Humanas |
| Beneficiário: | Luciano Puzer |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |