| Texto completo | |
| Autor(es): |
Almeida, Tuanny Leite
;
Moreira, Aline Francisca
;
de Oliveira, Jhones Luiz
;
Rogerio, Carolina Barbara
;
Kiihl, Samara Flamini
;
Fraceto, Leonardo Fernandes
;
de Jesus, Marcelo Bispo
Número total de Autores: 7
|
| Tipo de documento: | Artigo Científico |
| Fonte: | TOXICOLOGY IN VITRO; v. 95, p. 9-pg., 2023-12-07. |
| Resumo | |
The incidence of viruses such as Zika, Dengue, and Chikungunya affects human health worldwide, and insect repellents are recommended for individual protection. Formulations incorporating nanotechnology should be carefully assessed for toxicity, particularly regarding the security levels established for human health and the environment. This study evaluates the cytotoxicity of a repellent formulation containing zein nanoparticles (NP) loading geraniol (Ger) and icaridin (Ica) in three cell lines: NIH/3T3, HaCaT, and SIRC. To address formulation hazards, IC50 values were determined by MTT and Calcein-AM assays. In both NIH/3T3 and HaCaT, the IC50 values for NP + Ger + Ica formulation were around 0.2%. For risk assessment, cell viability was also determined after a single exposure and repeated exposure to the formulation. No evidence of cytotoxicity was observed for NP + Ger + Ica formulation-treated cells. The risk assessment for eye damage revealed cytotoxicity in SIRC cells when exposed to a 5% concentration, which may be attributed to ocular geraniol toxicity, because zein nanoparticles alone did not exhibit any signs of toxicity. Cell internalization indicated low uptake in NIH/3T3 and HaCaT cells. Phenotypic profiling resulted in similar phenotypes for untreated cells and cells exposed to NP + Ger + Ica formulation. The toxicological profile outlined by the multiparametric and orthogonal approach suggests that the NP + Ger + Ica formulation poses no significant risk to the topical application under the tested conditions. Adopting an orthogonal approach brings robustness to our findings. (AU) | |
| Processo FAPESP: | 20/01218-3 - Nanotoxicidade analisada individualmente através de perfil multiparamétrico baseada em imagem |
| Beneficiário: | Marcelo Bispo de Jesus |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 15/06134-4 - EMU concedido no processo 2014/03002-7: leitor multi-modo de imageamento celular (cell imaging multi-mode reader) |
| Beneficiário: | Marcelo Bispo de Jesus |
| Modalidade de apoio: | Auxílio à Pesquisa - Programa Equipamentos Multiusuários |
| Processo FAPESP: | 14/03002-7 - Internalização e tráfego intracelular de nanopartículas: atividade biológica e perfil nanotoxicológico |
| Beneficiário: | Marcelo Bispo de Jesus |
| Modalidade de apoio: | Auxílio à Pesquisa - Jovens Pesquisadores |