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Characterization of packed-bed in the downcomer of a concentric internal-loop airlift bioreactor

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Autor(es):
Mazziero, Vitor Teixeira ; Batista, Vinicius Guerso ; de Oliveira, Daniele Goncalves ; Scontri, Mateus ; de Paula, Ariela Veloso ; Cerri, Marcel Otavio
Número total de Autores: 6
Tipo de documento: Artigo Científico
Fonte: Biochemical Engineering Journal; v. 181, p. 9-pg., 2022-04-01.
Resumo

A packed-bed basket containing corn cob powder was used for the adaptation of a concentric-tube airlift reactor for enzymatic reactions. The bed was inserted in the downcomer region of the bioreactor generating a decrease in liquid downflow. To characterize the liquid velocity through the packed-bed and calculate the residence time, the intrinsic permeability was determined using Darcy's law, resulting in 1.275 x 10(-9) m(2) (40-mesh particle size), and the pressure difference was calculated using the riser gas holdup, reaching values up to 56.6% for water at 3.5 vvm. Thus, a superficial liquid velocity model was obtained to describe the liquid velocity at different airflow rates and bed lengths, covering a wide range of support masses commonly used in enzymatic reactions. The model also allows the variation of viscosity, caused by different reaction temperatures and the use of larger bed sizes for scaling. Using the dye tracer method, it was possible to visualize the flow through the bed and corroborate the residence times obtained by the model for a wide range of airflows. Therefore, it describes the use of a basket filled with potential enzyme support, contributing to the applicability of pneumatic systems as enzymatic bioreactors, particularly internal-loop airlift reactors. (AU)

Processo FAPESP: 20/08699-7 - Melhoria de desempenho em biorreator Airlift de circulação interna através de modificações geométricas no tubo interno
Beneficiário:Marcel Otavio Cerri
Modalidade de apoio: Auxílio à Pesquisa - Regular
Processo FAPESP: 17/11482-7 - Síntese enzimática de triglicerídeos dietéticos empregando reatores do tipo Airlift, leito fixo e leito fluidizado
Beneficiário:Ariela Veloso de Paula
Modalidade de apoio: Auxílio à Pesquisa - Regular
Processo FAPESP: 20/09592-1 - Ésteres bioativos nanoencapsulados: síntese enzimática e estudo do potencial antimicrobiano
Beneficiário:Ariela Veloso de Paula
Modalidade de apoio: Auxílio à Pesquisa - Regular