Zone trapping/merging zones in flow analysis: A novel approach for rapid assays involving relatively slow chemical reactions

Autor(es)

Vida, Ana C. F. ^[1] ; Sasaki, Milton K. ^[1] ; Gomes, Taciana F. ^[1] ; Silva, Claudineia R. ^[1] ; Feres, Mario A. ^[1] ; Zagatto, E. A. G. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Ctr Nucl Energy Agr, BR-13400970 Piracicaba - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Talanta; v. 85, n. 1, p. 259-263, JUL 15 2011.
• Citações Web of Science: 6

Resumo

A novel strategy for accomplishing zone trapping in flow analysis is proposed. The sample and the reagent solutions are simultaneously inserted into convergent carrier streams and the established zones merge together before reaching the detector, where the most concentrated portion of the entire sample zone is trapped. The main characteristics, potentialities and limitations of the strategy were critically evaluated in relation to an analogous flow system with zone stopping. When applied to the spectrophotometric determination of nitrite in river waters, the main figures of merit were maintained, exception made for the sampling frequency which was calculated as 189h(-1), about 32% higher relatively to the analogous system with zone stopping. The sample inserted volume can be increased up to 1.0 mL without affecting sampling frequency and no problems with pump heating or malfunctions were noted after 8-h operation of the system. In contrast to zone stopping, only a small portion of the sample zone is halted with zone trapping, leading to these beneficial effects. (C) 2011 Elsevier B.V. All rights reserved. (AU)

• Processo FAPESP: 10/00972-4 - Exploração de fluxos pulsados e difusão gasosa em sistema polivalente de análises em fluxo: monitoramento de sulfeto, sulfito e etanol durante a fermentação alcoólica.
• Beneficiário: Claudineia Rodrigues da Silva
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 06/07309-3 - Novas estratégias analíticas para estudos envolvendo biomoléculas
• Beneficiário: Elias Ayres Guidetti Zagatto
• Modalidade de apoio: Auxílio à Pesquisa - Temático