| Texto completo | |
| Autor(es): |
Gomes, G. S.
;
Benin, T.
;
Ferreira, E. C. J.
;
Palma, V. M.
;
Alves, A. C. S.
;
Visioli, F.
;
Longhi, M. S.
;
Pohlmann, A. R.
;
Guterres, S. S.
;
Frank, L. A.
Número total de Autores: 10
|
| Tipo de documento: | Artigo Científico |
| Fonte: | JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY; v. 99, p. 13-pg., 2024-07-24. |
| Resumo | |
This study aimed to develop a novel pectin-based hydrogel (PEC) incorporating nanocapsules-loaded tacrolimus (NCtac), named PEC-NCtac, as a potential strategy for psoriasis treatment. Blank nanocapsules (NC), PEC, PECNC, non-encapsulated tacrolimus (TAC), and the commercial TAC ointment (Tacroz (R)) were used as controls. The objectives involved physicochemical characterizing PEC-NCtac, assessing its cytotoxicity and anti-inflammatory effects, comparing adhesiveness with controls, determining skin penetration and permeation, and evaluating irritation potential. PEC-NCtac had an unimodal size distribution by laser diffraction (mean diameter of 250 nm and polydispersity -SPAN- of 1.68), drug content of 0.81 mg g- 1 (HPLC), and pH of 4.51 (potentiometry). The nanoformulation had no significant impact on the viability of HaCat cells and CD4+ T cells when tested 24 h after treatment, with both cell types maintaining nearly 100% viability. Treatment with NCtac resulted in a reduction in the pro-inflammatory cytokine IL-17A (p < 0.05). Adhesion studies revealed that PEC-NC showed a 1.9-fold higher force work compared to PEC. The combined effect of individual adhesion of NC and PEC when associated (PEC-NC) was evident in both healthy and injured skin (Healthy skin = 73 mN mm; Injured skin = 251 mN mm). PEC-NCtac allowed a decrease in skin drug permeation in the epidermis of injured skin compared to Tacroz (R) (40 % vs. 70 %, respectively). PEC-NCtac exhibited non-irritating activity in the HET-CAM/CAM-TBS model, while TAC demonstrated slight irritant activity. Furthermore, PEC-NCtac showed superior anti-inflammatory effects compared to TAC (75.0 % vs. 58 %, P value = 0.03). Summarizing, PEC-NCtac appears promising for topical treatment, demonstrating enhanced adhesion, skin penetration, safety profile, and anti-inflammatory activity. (AU) | |
| Processo FAPESP: | 14/50928-2 - INCT 2014: Nanotecnologia Farmacêutica: uma abordagem transdisciplinar |
| Beneficiário: | Maria Vitória Lopes Badra Bentley |
| Modalidade de apoio: | Auxílio à Pesquisa - Temático |