| Texto completo | |
| Autor(es): Mostrar menos - |
Martini, Tatiana
;
Camara, Joao Victor Frazao
;
Dionizio, Aline
;
Ventura, Talita Mendes Oliveira
;
Cassiano, Luiza de Paula Silva
;
de Souza e Silva, Cintia Maria
;
Taira, Even Akemi
;
Araujo, Tamara Teodoro
;
Santos, Lethycia Almeida
;
Ferrari, Carolina Ruis
;
Magalhaes, Ana Carolina
;
Carvalho, Thiago Saads
;
Baumann, Tommy
;
Lussi, Adrian
;
Rios, Daniela
;
de Oliveira, Ricardo Brandt
;
Palma-Dibb, Regina Guenka
;
Buzalaf, Marilia Afonso Rabelo
Número total de Autores: 18
|
| Tipo de documento: | Artigo Científico |
| Fonte: | Journal of Dentistry; v. 139, p. 7-pg., 2023-10-14. |
| Resumo | |
Objective: To evaluate the difference in the proteomic profile of stimulated saliva in patients with gastroesophageal reflux disease (GERD) with (GE) and without (GNE) erosive tooth wear (ETW), regarding both human and bacterial proteins.Methods: Stimulated saliva (SS) was collected from 16 patients (8/group). Samples were centrifuged at 4.500 g for 15 min under refrigeration to remove all debris. The supernatant from each saliva sample was taken and frozen at -80 degrees C. After extracting the proteins, they were submitted to reverse phase liquid chromatography and mass spectrometry (nLC-ESI-MS/MS). Label-free proteomic quantification was performed using Protein Lynx Global Service (PLGS) software (p < 0.05) for human and bacterial proteins.Results: In total, 67 human proteins were common for GNE and GE groups. GNE group presented, compared to GE group, increase in proteins that confer antimicrobial and acid resistant properties, such as cystatins, histatin and immunoglobulins. However, GNE group had a marked decrease in subunits of hemoglobin (alpha, beta and delta). Regarding bacterial proteins, for SS, 7 and 10 unique proteins were identified in the GE and GNE groups, respectively. They are related to protein synthesis and energy metabolism and interact with human proteins typically found in saliva and supramolecular complexes of the acquired pellicle.Conclusions: Our data indicate that the stimulation of the salivary flow increases acid resistant and antimicrobial proteins in saliva, which might protect against ETW. (AU) | |
| Processo FAPESP: | 17/17977-8 - Proteoma da película adquirida e saliva em voluntários com refluxo gastresofágico com erosão dentária ou não e avaliação in vitro do potencial protetor da hemoglobina contra a erosão dentária |
| Beneficiário: | Tatiana Martini |
| Modalidade de apoio: | Bolsas no Brasil - Doutorado |
| Processo FAPESP: | 18/12041-7 - Segurança e eficácia para prevenção da erosão dentária de uma nova cistatina derivada da cana-de-açúcar (CaneCPI-5): estudos in vitro, in situ e in vivo |
| Beneficiário: | Marília Afonso Rabelo Buzalaf |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |