| Texto completo | |
| Autor(es): |
Meira, Caroline Munhoz
;
Carriero, Mateus Maldonado
;
Pereira, Nycolas Levy
;
Rihs, Pedro Gustavo Macedo
;
Lazaro, Talita Maria
;
Rocha, Nathalia Raissa Alcantara
;
Maia, Antonio Augusto Mendes
Número total de Autores: 7
|
| Tipo de documento: | Artigo Científico |
| Fonte: | JOURNAL OF FISH DISEASES; v. 47, n. 9, p. 13-pg., 2024-06-16. |
| Resumo | |
The increasing significance of the aquaculture sector and commercially valuable species underscores the need to develop alternatives for controlling diseases such as Ichthyophthirius multifiliis-induced ichthyophthiriasis. This ciliated protozoan parasite threatens nearly all freshwater fish species, causing substantial losses in the fishery industry. Despite this, effective large-scale treatments are lacking, emphasizing the necessity of adopting preventive strategies. While the pathogenesis of ichthyophthiriasis and its immune stimulation allows for vaccination strategies, precise adjustments are crucial to ensure the production of an effective vaccine compound. Therefore, this study aimed to evaluate the impact of immunizing Astyanax lacustris with a genetic vaccine containing IAG52A from I. multifiliis and the molecular adjuvant IL-8 from A. lacustris. Transcript analysis in immunized A. lacustris indicated mRNA production in fish muscles, demonstrating an expression of this mRNA. Fish were divided into five groups, receiving different vaccine formulations, and all groups received a booster dose 14 days after the initial immunization. Samples from vaccinated fish showed increased IL-1 beta mRNA expression in the spleen within 6 h post the second dose and after 14 days. In the head kidney, IL-1 beta mRNA expression showed no significant difference at 6 and 24 h but an increase was noted in fish injected with IAG and IAG + IL-8 after 14 days. IL-8 mRNA expression in the spleen and kidney did not significantly differ from the control group. Histological analysis revealed no variation in leukocyte concentration at 6 and 24 h post-vaccination; however, after 14 days, the groups injected with IAG and IAG + IL-8 exhibited a higher leukocyte density at the application sites than the control. The obtained data suggest that the used vaccine is transcribed, indicating its potential to stimulate innate immune response parameters through mRNA cytokine expression and leukocyte migration. (AU) | |
| Processo FAPESP: | 17/26996-6 - Avaliação da triploidia na susceptibilidade à infecção por Aeromonas jandaei e possível relação com os níveis de expressão de genes de resposta imune em lambaris (Astyanax altiparanae) |
| Beneficiário: | Antonio Augusto Mendes Maia |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 20/06371-4 - Efeito da imunização de lambaris (Astyanax altiparanae) com o antígeno de imobilização IAG52A de Ichthyophthirius multifiliis associado com a citocina IL-8 de A. altiparanae como adjuvante molecular |
| Beneficiário: | Caroline Munhoz Meira |
| Modalidade de apoio: | Bolsas no Brasil - Mestrado |
| Processo FAPESP: | 17/23573-7 - Caracterização e avaliação dos efeitos da triploidia na expressão dos genes das citocinas IL-1b, IFNy e TGFb e das enzimas Arg2 e iNOS em lambaris (Astyanax altiparanae)" |
| Beneficiário: | Caroline Munhoz Meira |
| Modalidade de apoio: | Bolsas no Brasil - Iniciação Científica |