Dental caries experience in relation to salivary findings and molecular identification of S. mutans and S. sobrinus in subjects with Down syndrome

Autor(es)

Freire de Castilho, Aline Rogeria ^[1] ; Pardi, Vanessa ^[2] ; Pereira, Cassio Vicente ^[3]

Afiliação do(s) autor(es)

^[1] Univ Campinas UNICAMP, Pedodont Div, Dept Pediat Dent, Piracicaba Dent Sch, BR-13414903 Piracicaba, SP - Brazil

^[2] Univ Campinas UNICAMP, Dept Community Dent, Piracicaba Dent Sch, BR-13414903 Piracicaba, SP - Brazil

^[3] Univ Campinas UNICAMP, Dept Microbiol & Immunol, Piracicaba Dent Sch, BR-13414903 Piracicaba, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: ODONTOLOGY; v. 99, n. 2, p. 162-167, JUL 2011.
• Citações Web of Science: 5

Resumo

This study investigated the association between clinical and salivary or molecular parameters in Down syndrome subjects. Sixty individuals (1- to 48-year old) were clinically examined using DMFT/DMFS. Stimulated saliva was collected; salivary flow was calculated (mL/min), buffering capacity was measured using a standard pH tape. In addition, 25 mu L of saliva was diluted using 10-fold-dilution method and then placed on Mitis-Salivarius-Bacitracin agar to count colony forming units (CFU/mL) of mutans streptococci. Polymerase chain reaction analysis identified species. Caries indexes were 0.65-13.5 (DMFT) and 0.65-26.0 (DMFS) according to groups. Ninety-four percent of subjects had low flow rate (0.7-1.0 mL/min) and 44% had low buffering capacity (pH < 4). Besides, 60% had more than 1 x 10(6) CFU/mL, 60% had S. mutans, and 41.4% had S. sobrinus. Caries indexes did not significantly correlate with flow rate, buffering capacity, CFU/mL by Pearson's correlation (p > 0.05), and showed no significant association with prevalence of species by Chi-square (p > 0.05). There is no association between clinical picture and salivary or molecular parameters in Down syndrome subjects. (AU)

• Processo FAPESP: 04/12925-0 - Deteccao molecular de streptococcus mutans e streptococcus sobrinus e avaliacao de parametros clinicos e salivares em pacientes com sindrome de down.
• Beneficiário: Aline Rogéria Freire de Castilho
• Modalidade de apoio: Bolsas no Brasil - Mestrado